Project description:Serum of LCMV infected mice. Data was generated on a Thermo Q Exactive and C18 RP UHPLC. Positive polarity acquisition on LC-MS/MS.
Project description:Update of dataset of serum of LCMV infected mice. No Phree Kit used in extraction protocol. Data was generated on a Thermo Q Exactive and C18 RP UHPLC. Positive polarity acquisition on LC-MS/MS.
Project description:We used data independent acquisition (DIA) mass spectrometry (MS) to profile ~800 proteinsfrom 122 serum samples Dengue or Zika Trinidadian patients. Two time points were collectedper patient. The DIA MS data were matched against a spectral library generated from high pH/low pH separated pooled serum samples.
Project description:Wildtype mice and Autotaxin-overexpressing transgenic mice, both with and without spontaneous tumor formation, were maintained in a colony. Serum samples from mice in the aforementioned three groups were collected and a random subset of mice were selected for microarray analysis. RNA was extracted from serum and subsequently cDNA was generated and run on two 384-well plates probing for 373 viable targets, including microRNAs, control probes and interplate calibrators. Raw Ct values obtained from qPCR analysis were analyzed using GenEx and graphed using GraphPad Prism.
Project description:Metabolite analysis of DDW fecal samples, standard methanol extraction. Data were acquired using a Bruker Daltonics maXis Impact and C18 RP-UHPLC. Positive polarity acquisition of LC-MS/MS.
Project description:A miRNA microarray was performed from HCV infected patient serum samples of bothe genotype 1b and genotype 3a, which are prevalent in India, with the aim of identifying a set of miRNAs which are uniquely differentially expressed during HCV infection. miR-320c, miR-483-5p, miR-134 and miR-198 were found to be upregulated in the patient samples as compared to the controls and are currenty being validated.
Project description:Investigation of small molecules detected in bacterial inhibitory zone. Data were generated on a Thermo Q Exactive and C18 RP UHPLC. Positive polarity acquisition on LC-MS/MS.
Project description:Samples of cotton leaves, fall armyworm midgut content and faeces. Data was generated on a Thermo Q Exactive and C18 RP UPLC. Positive polarity acquisition on LC-MS/MS
Project description:Samples of cotton leaves, fall armyworm midgut content and faeces. Data was generated on a Thermo Q Exactive and C18 RP UPLC. Negative polarity acquisition on LC-MS/MS
Project description:Retained placenta (RP), a quite common disorder in dairy cows, shows a high negative impact on their health status and milk production. Therefore, the biomarkers indicating the risk for RP could yield benefits in dairy management. The study aimed to investigate the serum proteome as the source of the biomarkers for diagnosing the RP. Using the high-resolution liquid chromatography-tandem mass spectrometry, we compared the composition of serum proteome between 9 cows with the RP and 6 with the physiologic puerperium (PP). Among 651 identified proteins, seven had higher, and three showed lower abundance in RP than in the PP group. The differently abundant proteins participated in 15 pathways: six related to hemostasis, three involved in the lipoprotein metabolism, and the remaining ones associated with redox homeostasis, post-translational modification, scavenging, etc. Validation of the proteomic results, using ELISA and spectrophotometric assays, showed that the two identified proteins, haptoglobin, and lipopolysaccharide-binding protein, could represent reliable RP biomarkers. Our results confirmed that the analysis of serum proteome shed "the light" on the counterparty piece(s) of the RP pathogenesis "puzzle" and could be an efficient "tool" in mining for RP biomarkers.