Dataset Information

GNPS - high fiber diet mice methanol extracts

ABSTRACT: comparison between high fiber diet and ctrl mice methanol extraction, q exactive hf, 28 min method

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Mus Sp. (ncbitaxon:10095)

SUBMITTER: Frank Schroeder

PROVIDER: MSV000086890 | GNPS | Tue Feb 16 14:24:00 GMT 2021

REPOSITORIES: GNPS

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Publications

Inulin fibre promotes microbiota-derived bile acids and type 2 inflammation.

Arifuzzaman Mohammad M Won Tae Hyung TH Li Ting-Ting TT Yano Hiroshi H Digumarthi Sreehaas S Heras Andrea F AF Zhang Wen W Parkhurst Christopher N CN Kashyap Sanchita S Jin Wen-Bing WB Putzel Gregory Garbès GG Tsou Amy M AM Chu Coco C Wei Qianru Q Grier Alex A Worgall Stefan S Guo Chun-Jun CJ Schroeder Frank C FC Artis David D

Nature 20221102 7936

Dietary fibres can exert beneficial anti-inflammatory effects through microbially fermented short-chain fatty acid metabolites<sup>1,2</sup>, although the immunoregulatory roles of most fibre diets and their microbiota-derived metabolites remain poorly defined. Here, using microbial sequencing and untargeted metabolomics, we show that a diet of inulin fibre alters the composition of the mouse microbiota and the levels of microbiota-derived metabolites, notably bile acids. This metabo ...[more]

PMID: 36323778

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Project description:The prevalence of the metabolic syndrome (MS) is rapidly increasing all over the world. Consequently, there is an urgent need for more effective intervention strategies. Both animal and human studies indicate that lipid oversupply to skeletal muscle can result in insulin resistance which is one of the charecteristices of the MS. C57BL/6J mice were fed a low fat (10 kcal%) palm oil diet or a high fat (45 kcal%; HF) palm oil diet for 3 or 28 days. By combining transcriptomics with protein and lipid analyses we aimed to better understand the molecular events underlying the early onset of the MS. Short-term HF-feeding led to altered expression levels of genes involved in a variety of biological processes including morphogenesis, energy metabolism, lipogenesis and immune function. Protein analysis showed increased levels of the myosin heavy chain, slow fiber type protein and the complexes II, III, IV and V of the oxidative phosphorylation. Furthermore, we observed that the main mitochondrial membrane phospholipids, phosphatidylcholine and phosphatidylethanolamine, contained more saturated fatty acids. Altogether, these results point to a morphological as well as a metabolic adaptation by promoting a more oxidative fiber type. We hypothesize that after this early adaptation, a continued transcriptional down-regulation of genes involved in oxidative phosphorylation will result in decreased oxidative capacity at a later stage. Together with increased saturation of phospholipids of the mitochondrial membrane this can result in decreased mitochondrial function which is a hallmark observed in insulin resistance and type 2 diabetes. Keywords: diet intervention and time course In the present study we investigated the short versus the long term effects of a high fat diet on the mouse muscle transcriptome by performing a genome-wide analysis of high fat diet-responsive genes. C57BL/6J mice were fed a low fat (LF) diet (10 kcal% palm oil) or a high fat (HF) diet (45 kcal% palm oil) for 3 or 28 days. Total RNA samples (n = 6 per diet per time point) were individually hybridised tot the Affymatrix mouse genome 430 2.0 array.