Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

GNPS Data to analyze the Lipidome of Mycobacterium tuberculosis H37Rv grown in zinc-replete and zinc-limited conditions

ABSTRACT: to analyze the lipidomes of Mycobacterium tuberculosis H37Rv grown in zinc-replete and zinc-limited conditions

INSTRUMENT(S): 6545 Q-TOF LC/MS

ORGANISM(S): Mycobacterium Tuberculosis H37rv (ncbitaxon:83332)

SUBMITTER: Philip G. Williams

PROVIDER: MSV000087030 | GNPS | Wed Mar 10 15:59:00 GMT 2021

REPOSITORIES: GNPS

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Transcriptional response in RAW 264.7 macrophages infected with zinc-replete and zinc-limited Mycobacterium tuberculosis

Project description:The goal of this experiment was to determine if macrophages can distinguish between M. tuberculosis grown in standard, zinc-replete media and those grown in zinc-limited media. In order to determine this, we performed RNA-sequencing (RNA-seq) of RAW 264.7 macrophages infected with zinc-limited Mtb and zinc-replete Mtb at 4 hours post-infection (hpi) and 24hpi. Transcriptional response in the infected macrophages was compared to uninfected controls (macrophages exposed to growth media alone) and between macrophages infected with zinc-limited Mtb and zinc-replete Mtb at each time point.

2025-01-17 | GSE255565 | GEO

Proteomics of Zinc-limited Mycobacterium tuberculosis

Project description:Mycobacterium tuberculosis H37Rv (Mtb) grown in chemically defined medium replete for Zinc and limited for Zinc

2021-04-23 | PXD024389 | Pride

Transcriptomics of Zinc-limited and Zinc-replete Mycobacterium tuberculosis

Project description:Transcriptomics of Zinc-limited and Zinc-replete Mycobacterium tuberculosis

| PRJNA707587 | ENA

Macrophage responses to zinc-limited and zinc-replete Mycobacterium tuberculosis H37Rv

Project description:The goal of this experiment was to determine if macrophage responses differ between macrophages infected with M. tuberculosis H37Rv grown in the standard, zinc-replete medium compared to cells grown in zinc-limited medium. The macrophage responses after 4 hours and 24 hours of infection were determined by isolating the RNA and analyzed it with the NanoString panel mouse host response panel.

2025-01-17 | GSE256252 | GEO

Macrophage responses to zinc-limited and zinc-replete Mycobacterium tuberculosis H37Rv

Project description:Macrophage responses to zinc-limited and zinc-replete Mycobacterium tuberculosis H37Rv

| PRJNA1078784 | ENA

RNA-seq of zinc-replete wild type and zinc-limited wild type, altRP operon deletion mutant and its complement strain in Mycolicibacterium (Mycobacterium) smegmatis

Project description:The study was designed to describe the effects of zinc limitation on gene expression in M. smegmatis by comparing the transcriptomes of zinc-replete and zinc-limited wild type. Additionally, the study informs how loss of the altRP operon impacts the transcriptomic changes during zinc limitation by comparing the transcriptomes of zinc-limited ΔaltRP mutant to the zinc-replete and zinc-limited wild type, and to confirm which changes are complemented in the complement strain (ΔaltRP/c).

2021-11-10 | GSE188233 | GEO

Transcriptome response to carbon-limiting conditions.

Project description:Comparison of cells grown under carbon-limited and carbon-replete conditions.

2020-06-01 | GSE147051 | GEO

A conserved nucleotide-dependent metal chaperone for managing the cellular zinc economy during zinc limitation

Project description:How cells safeguard essential zinc-dependent functions during zinc deficiency is poorly understood. A long-debated strategy is whether soluble metal-trafficking chaperones exist to prioritize specific zinc-dependent proteins. We identified a eukaryotic family of metallochaperones that physically interacts with zinc-dependent methionine aminopeptidase type I (MAP1) in human and yeast. Deletion of the yeast metallochaperone-encoding gene NMC1 (formerly YNR029c) leads to a zinc-deficiency growth defect and defective initiator methionine cleavage caused by loss of Map1p activity. To better understand the observed fitness defects due to the lack of NMC1 under zinc deficiency, we used proteomics with Tandem Mass Tag (TMT) quantitation derived from WT, nmc1Delta, and map2Delta nmc1Delta strains grown in zinc-limited (1 uM) or zinc-replete (100 uM) conditions. Proteomics reveal global impacts due to the loss of NMC1 and Map1p function, including mis-regulation of the Zap1p regulon, and suggests that Nmc1p is required to avoid a compounding effect of Map1p dysfunction on cell survival during zinc deficiency.

| MSV000088135 | MassIVE

Shotgun proteomics (qMS) of zinc-replete wild type and zinc-limited wild type, altRP operon deletion mutant and its complement strain in Mycolicibacterium (Mycobacterium) smegmatis

Project description:The study was designed to describe the effects of zinc limitation on gene expression in M. smegmatis by comparing the proteomes of zinc-replete and zinc-limited wild type. Additionally, the study informs how loss of the altRP operon impacts the proteome during zinc limitation by comparing the proteomes of zinc-limited ΔaltRP mutant to the zinc-replete and zinc-limited wild type, and to confirm which changes are complemented in the complement strain (ΔaltRP/c).

2021-11-10 | PXD029588 | Pride

RNA-seq of suspension-grown vs. adherent Neisseria gonorrhoeae under conditions of zinc sequestration or zinc excess

Project description:RNA-seq of suspension-grown vs. adherent Neisseria gonorrhoeae under conditions of zinc sequestration or zinc excess

| PRJNA789354 | ENA