Project description:The goal of this experiment was to determine if macrophages can distinguish between M. tuberculosis grown in standard, zinc-replete media and those grown in zinc-limited media. In order to determine this, we performed RNA-sequencing (RNA-seq) of RAW 264.7 macrophages infected with zinc-limited Mtb and zinc-replete Mtb at 4 hours post-infection (hpi) and 24hpi. Transcriptional response in the infected macrophages was compared to uninfected controls (macrophages exposed to growth media alone) and between macrophages infected with zinc-limited Mtb and zinc-replete Mtb at each time point.
Project description:The study was designed to describe the effects of zinc limitation on gene expression in M. smegmatis by comparing the transcriptomes of zinc-replete and zinc-limited wild type. Additionally, the study informs how loss of the altRP operon impacts the transcriptomic changes during zinc limitation by comparing the transcriptomes of zinc-limited ΔaltRP mutant to the zinc-replete and zinc-limited wild type, and to confirm which changes are complemented in the complement strain (ΔaltRP/c).
Project description:The goal of this experiment was to determine if macrophage responses differ between macrophages infected with M. tuberculosis H37Rv grown in the standard, zinc-replete medium compared to cells grown in zinc-limited medium. The macrophage responses after 4 hours and 24 hours of infection were determined by isolating the RNA and analyzed it with the NanoString panel mouse host response panel.
Project description:The study was designed to describe the effects of zinc limitation on gene expression in M. smegmatis by comparing the proteomes of zinc-replete and zinc-limited wild type. Additionally, the study informs how loss of the altRP operon impacts the proteome during zinc limitation by comparing the proteomes of zinc-limited ΔaltRP mutant to the zinc-replete and zinc-limited wild type, and to confirm which changes are complemented in the complement strain (ΔaltRP/c).
Project description:Klebsiella pneumoniae is a bacterial pathogen that causes nosocomial infection in humans and is acquiring antibiotic resistance at an alarming rate. This study investigates the effect of zinc limitation on the phosphoproteome of K. pneumoniae using quantitative mass spectrometry to provide insight into the cell signaling methods used to respond to nutrient limited conditions like those experienced when colonizing a host.
Project description:Investigation of whole genome gene expression level changes in a Nitrosomonas europaea (ATCC 19718) wildtype and pFur::Kan mutant [kanamycin resistance cassette insertion in the promoter region of the fur gene (NE0616)] strains grown in Fe-replete and Fe-limited media. The Nitrosomonas europaea (ATCC 19718) wiltype cells grown in Fe-limited media were compared to cells grown in Fe-replete media to gain a better understanding of the metabolic changes occurring in response to iron stress. The Nitrosomonas europaea (ATCC 19718) pFur::Kan mutant strain grown in Fe-replete & Fe-limited media were compared to wildtype cells grown in Fe=replete & Fe-limited media to gain a better understanding of the role Fur (NE0616) plays in iron homeostasis control.