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GNPS DOM Interlab Ring Trial LCMS - Lab 26

ABSTRACT: DOM Interlab LCMS Samples from Lab 26. Infused with a Thermo Dionex 3000 RSLC using the loading pump at 400 ul/min with gradient as prescribed onto a Hypersil Gold C18 column (2.1x100mm, 1.9um particle size). Analysed with a Thermo Orbitrap Exploris 480 using HESI source in positive and negative mode with MS1 and MS2 methods. Note: sample was used up prematurely so duplicate injection replicates only for Pos MS1 and MS2, and neg MS2. Negative MS1 had triplicate injections. Internal calibration (IC) source enabled for improved mass accuracy. Sample infusion order: Neg MS1 rep 1, Neg MS2 rep 2, Pos MS1 rep 1, Pos MS2 rep1, (and then repeated). Within each polarity/MS group, samples were randomised - and differently between each group. No-injection blanks were run between group, and QCs and solvent and PPL SPE blanks were analyzed before the first rep of each polarity/MS setting.

INSTRUMENT(S): Orbitrap Exploris 480

ORGANISM(S): Synechococcus (ncbitaxon:1129) Environmental Samples <bacillariophyta> (ncbitaxon:33858)

SUBMITTER: Will Kew

PROVIDER: MSV000087935 | GNPS | Tue Aug 03 00:34:00 BST 2021

REPOSITORIES: GNPS

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