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GNPS Multimodal Mass Spectrometry Imaging of Rat Brain using IR-MALDESI and nanoPOTS-LC-MS/MS

ABSTRACT: Multimodal mass spectrometry imaging (MSI) is a critical technique used for deeply investigating biological systems by combining multiple MSI platforms in order to gain the maximum molecular information about a sample that would otherwise be limited by a single analytical technique. The aim of this work was to create a multimodal MSI approach that measures metabolomic and proteomic data from a single biological organ by combining infrared matrix-assisted laser desorption electrospray ionization (IR-MALDESI) for metabolomic MSI and nanodroplet processing in one pot for trace samples (nanoPOTS) LC-MS/MS for spatially resolved proteome profiling. Adjacent tissue sections of rat brain were analyzed by each platform and each dataset was individually analyzed using previously optimized workflows. IR-MALDESI datasets were annotated by accurate mass and spectral accuracy using HMDB, METLIN, and LipidMaps databases, while nanoPOTS-LC-MS/MS datasets were searched against the rat proteome using the Sequest HT algorithm and filtered with a 1% FDR. The combined data revealed complementary molecular profiles distinguishing the corpus collosum against other sampled regions of the brain. A multiomic pathway integration showed a strong correlation between the two datasets when comparing average abundances of metabolites and corresponding enzymes in each brain region. This work demonstrates the first steps in the creation of a multimodal MSI technique that combines two highly sensitive and complementary imaging platforms.

INSTRUMENT(S): Q Exactive Plus, Orbitrap Exploris 480

ORGANISM(S): Rattus Norvegicus (ncbitaxon:10116)

SUBMITTER: David C. Muddiman

PROVIDER: MSV000088211 | GNPS | Mon Oct 11 12:16:00 BST 2021

REPOSITORIES: GNPS

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