Project description:Conditions of low organic matter content per gram of soil in the hyperarid core of the Atacama Desert, extreme temperatures and high UV radiation, makes it one of the best terrestrial analogue of Mars and one of the best scenarios for testing instrumentation devoted to in situ planetary exploration. We have remotely and automatically operated the SOLID-LDChip, an antibody microarray-based sensor instrument, as part of a rover payload during the 2019 NASA’s ASTEP ARADS Mars drilling simulating campaign. SOLID was loaded with a robotic arm with samples collected down to 80 cm depth and, after sample analysis with LDChip, sent results to a remote science team, to be validated by “omics” techniques.
Project description:Apis mellifera syriaca is the native honeybee subspecies of Jordan and much of the Middle East. It expresses behavioral adaptations to a regional climate with very high temperatures, nectar dearth in summer, attacks of the Oriental wasp Vespa orientalis and in most cases it is resistant to varroa mites. The Thorax control sample of A. m. syriaca in this experiment was originally collected and stored since 2001 from Wadi Ben Hammad a remote valley in the southern region of Jordan. Using morphometric and Mitochondrial DNA markers it was proved that bees from this area had show higher similarity than other samples collected from the Middle East as represented by reference samples collected in 1952 by Brother Adam. The samples L1-L5 are collected from the National Center for Agricultural Research and Extension breading apiary which was originally established for the conservation of Apis mellifera syriaca. Goal was to use the genetic information in the breeding for varroa resistant bees and to determine the successfulness of this conservation program. Project funded by USAID-MERC grant number: TA-MOU-09-M29-075.
Project description:As part of the ENCODE consortium project (NIH, NHGRI), we conducted genome-wide location analysis for various transcription factors in various cell types. The long-term goal of this project is to determine the function of each DNA nucleotide in the genome. Measurement of transcription factor binding events is an important part of this effort. Here we measure genome-wide binding events for a variety of important transcriptional regulators. Keywords: ChIP-chip
Project description:RNAseq of CD4 cells from 60 female RA patients activated with aCD3 for 2 hours. Patients were collected crossectionally from the Rheumatology clinic during 2019-2020. Age md 64, range 23-76 years, Disease duration md 10, range 1-45 years.
Project description:RNAseq of CD14 cells from 60 female RA patients activated with LPS for 2 hours. Patients were collected crossectionally from the Rheumatology clinic during 2019-2020. Age md 64, range 23-76 years, Disease duration md 10, range 1-45 years.
Project description:Sputum cells collected before (visit 2) and after (visit 4) allergen challenge in asthma patients were isolated and RNA purified for analysis on gene expression arrays. Human subject recruitment part of NIH sponsored protocol as part of the Eosinophil Program Project Grant (PI: Dr. Nizar Jarjour)
Project description:As part of the PhenoGen Project (http://phenogen.org), RNA-Seq data has been collected from whole brain samples of two inbred strains that represent the parental strains from the HXB/BXH recombinant inbred panel, which is part of the Hybrid Rat Diversity Panel. Processed data were generated as part of manuscript, Aptardi predicts polyadenylation sites in sample-specific transcriptomes using high throughput RNA sequencing and DNA sequence.
Project description:Gene expression changes in the blood was studied by RNAseq; project SIG-2019 Results: Strong differential host reponse after infection with influenza virus compared to healthy controls
Project description:A siRNA screening using a commercially available membrane trafficking library identified Rab11 as a pro-viral host factor in motor neuron NSC34 cell line infected with EV-A71, a major causative agent of Hand, Foot and Mouth Disease (HFMD). This project aims at deciphering the role of Rab11 during EV-A71 infection by using various molecular virology and cell biology approaches. We also identified Rab11 interacting partners during EV-A71 infection by pulldown and mass spectrometry.
Project description:All-genome genotyping data from French wild boar populations could be useful for diversity studies in the wild boar species as well as for phylogeny studies with domestic pig populations. The data produced in this experiment concern 362 wild boars collected between 2013 and 2019 in various French departments. The biological samples that were collected were either blood samples or ear biopsies. Genomic DNA was extracted from cells after proteinase K lysis and ethanol precipitation. DNA was hybridized on the GeneSeek Genomic Profiler porcine beadchip (GGP70K HD Porcine Illumina) using Infinium technology. Fluorescence intensity data obtained for each Single Nucleotide Polymorphism were analyzed with GenomeStudio software to infer genotypes. In addition, the raw fluorescence data could be useful for Copy Number Variation studies.