Project description:Top-down Proteomics of Venom from Naja haje from the Berlin Zoo. 4 technical replicates from TCEP reduced crude venom and 1 replicate from native venom.
Project description:The genus Bothrops is responsible for most part of envenomation accidents in Brazil. Bothrops pubescens is an endemic and neglected species in the Brazilian Pampa Biome. The characterization of its venom is essential since there is no data about it and can be helpful in the discovery of active biomolecules and for a better understanding of its action. We used top-down (TDP), native top-down, and bottom-up proteomic (BUP) approaches to characterize the venom of B. pubescens. We were able to identify 89 protein groups with the BUP approach and 40 unique proteoforms with the TDP approach, demonstrating the similarities and peculiarities of B. pubescens venom. We also identified a dimeric L-amino acid oxidase with using native TDP. Here we present for the first time a bothropic venom characterization through TDP approaches.
Project description:Top-down proteomics of venom protein of venom-gland organoids aspidelaps. Samples were extracted with MilliQ water and proteins reduced with TCEP before top-down LC-MS/MS analysis.
Project description:Top-down proteomics of venom protein of venom-gland organoids aspidelaps. Samples were extracted with MilliQ water and proteins reduced with TCEP before top-down LC-MS/MS analysis.
Run 2
Project description:Venom proteomics analysis of Walterinnesia aegyptia and Walterinnesia morgani. For top-down analysis, venom samples were reduced with TCEP and measured via HPLC-MS/MS (Q-Exactive and LTQ-Orbitrap XL).