Project description:Top-down proteomics of venom protein of venom-gland organoids aspidelaps. Samples were extracted with MilliQ water and proteins reduced with TCEP before top-down LC-MS/MS analysis.
Project description:Top-down proteomics of venom protein of venom-gland organoids aspidelaps. Samples were extracted with MilliQ water and proteins reduced with TCEP before top-down LC-MS/MS analysis.
Run 2
Project description:Venom proteomics analysis of Walterinnesia aegyptia and Walterinnesia morgani. For top-down analysis, venom samples were reduced with TCEP and measured via HPLC-MS/MS (Q-Exactive and LTQ-Orbitrap XL).
Project description:Top-down Proteomics of Venom from Naja haje from the Berlin Zoo. 4 technical replicates from TCEP reduced crude venom and 1 replicate from native venom.
Project description:Top-down and bottom-up protein analysis of venom and saliva of solenodon. Venom and saliva was separated by HPLC and either directly analysis by HR FT MS/MS (Top-down) or further decomplexed by SDS-PAGE followed by in-gel trypsine digestion and HR LC-MS/MS analysis (bottom-up, Venom only). For shotgun bottom-up comparison of venom and saliva proteins, samples were directly reduced, alkylated, digested with trypsin and measured by HPLC-MS/MS. Additional bottom-up analysis was performed from bioactivity guided fractionation experiments.
Project description:we used a combination of bottom-up (BU) and top-down (TD) proteomics approaches to identify and characterize venom protein compositions of Echis carinatus sochureki (ECS) from three different Iranian populations.