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GNPS - Nanodisc Assembly E. coli and Brain Lipids

ABSTRACT: Data set for lipidomic analysis of nanodiscs assembled with different temperatures, belt sizes, and lipid types, including both E. coli and porcine brain polar lipid extracts.

INSTRUMENT(S): Synapt XS

ORGANISM(S): Escherichia Coli (ncbitaxon:562) Sus Scrofa Domesticus (ncbitaxon:9825)

SUBMITTER: Michael Marty

PROVIDER: MSV000091857 | GNPS | Thu May 04 10:16:00 BST 2023

REPOSITORIES: GNPS

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Publications

Do Nanodisc Assembly Conditions Affect Natural Lipid Uptake?

Odenkirk Melanie T MT Zhang Guozhi G Marty Michael T MT

Journal of the American Society for Mass Spectrometry 20230731 9

Lipids play critical roles in modulating membrane protein structure, interactions, and activity. Nanodiscs provide a tunable membrane mimetic that can model these endogenous protein-lipid interactions in a nanoscale lipid bilayer. However, most studies of membrane proteins with nanodiscs use simple synthetic lipids that lack the headgroup and fatty acyl diversity of natural extracts. Prior research has successfully used natural lipid extracts in nanodiscs that more accurately mimic natural envir ...[more]

PMID: 37524089

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