GNPS - NISTfecalRM_Astral_HypersilGoldC18_positive_2
Ontology highlight
ABSTRACT:
INSTRUMENT(S): Orbitrap Astral
ORGANISM(S): Homo Sapiens (ncbitaxon:9606)
SUBMITTER: 
Pieter C. Dorrestein
PROVIDER: MSV000093527 | GNPS | Tue Nov 28 22:07:00 GMT 2023
REPOSITORIES: GNPS
Similar Datasets
Project description:NIST fecal reference material extracted with 50% MeOH and prepared in different dilutions and reconstitution solvents. The analysis was conducted using a Hypersil Gold (C18) in negative ionization mode on a Astral via different MS-modes.
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Project description:NIST fecal reference material extracted with 50% MeOH and prepared in different dilutions and reconstitution solvents. The analysis was conducted using a Hypersil Gold (C18) in positive ionization mode on a Astral via different MS-modes (positive ionization mode).
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Project description:NIST fecal reference material extracted with 50% MeOH and prepared in different dilutions and reconstitution solvents. The analysis was conducted using a Hypersil Gold (C18) in positive ionization mode on a Astral via different MS-modes (positive ionization mode).
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Project description:The National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK) in collaboration with the National Institute of Standards (NIST) recently produced a human plasma standard reference material (SRM 1950) for metabolite analysis. The SRM was prepared by obtaining plasma samples from 100 individuals between 40 and 50 years of age, whose ethnicity was representative of the US population and that included an equal number of men and women. The intent of the NIDDK/NIST project was to provide a reference material that would be publically available to researchers and that could be used by the clinical chemistry community to identify plasma metabolites for diagnostic purposes. Signature metabolites could then be further probed for their usefulness as disease biomarkers. The LIPID MAPS Consortium has undertaken the task to analyze this SRM by systematically identifying and quantifying the lipid molecular species in the six main categories of mammalian lipids. The quantitative levels of over 500 different lipids present in this reference human plasma sample are presented here.
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Project description:Proper QA/QC is an essential component of any analytical procedure. The variability in platforms and approaches used by the untargeted cores requires that each lab develop specific QA/QC protocols, making cross-laboratory assessments challenging. To address the difference in measures across the untargeted cores, the untargeted quality assurance working group (UQAWG) has identified the importance of using the same biological reference sample across the different laboratories. To reach this goal, Dr. Jones and Mr. Walker of Emory University volunteered to take the lead in identifying vendors, requesting quotes, purchasing the pooled material, delivering the specified volumes to each of the lab hubs and storing additional material on-site at Emory University. 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Project description:Proper QA/QC is an essential component of any analytical procedure. The variability in platforms and approaches used by the untargeted cores requires that each lab develop specific QA/QC protocols, making cross-laboratory assessments challenging. To address the difference in measures across the untargeted cores, the untargeted quality assurance working group (UQAWG) has identified the importance of using the same biological reference sample across the different laboratories. To reach this goal, Dr. Jones and Mr. Walker of Emory University volunteered to take the lead in identifying vendors, requesting quotes, purchasing the pooled material, delivering the specified volumes to each of the lab hubs and storing additional material on-site at Emory University. 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Using reference standardization, quantification post-data acquisition is possible by referencing the pooled material analyzed with each series of samples. The pooled material can be characterized and analytes quantified using traditional analytical chemistry techniques, such as quantification by methods of addition or standardization with a certified reference material, such as NIST SRM 1950. Known concentrations within the pooled material can be used to determine an analyte response factor and calculate sample concentrations based on single-point calibration. The benefit of this approach is that targeted quantification is only required in the pooled sample (i.e. UrineRef_20160721 or PlasmaRef_20160726), chemicals selected for quantification do not need to be selected a priori, and population wide estimates of plasma chemical concentrations can be determined without having to re-analyze samples using a targeted approach. 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