Project description:The ultimate aim is see if rhizosphere microbiota are influenced by changes in root exudate composition resulting from abiotic stress. The abiotic variables we are focusing on at this stage are salinity, temperature and pH. This can be divided into two questions: (a) how do plant exudates change in response to abiotic stress, and (b) how do these changes influence bacteria. In order to test this we will produce plant exudates under controlled stressed conditions, measure their composition and measure bacterial growth in these exudates. Data has also been produced from synthetic community experiments comparing the community composition under a variety of controlled stress conditions (temperature, salinity, pH, and phosphate).
The work (proposal:https://doi.org/10.46936/10.25585/60000944) conducted by the U.S. Department of Energy Joint Genome Institute (https://ror.org/04xm1d337), a DOE Office of Science User Facility, is supported by the Office of Science of the U.S. Department of Energy operated under Contract No. DE-AC02-05CH11231.
Project description:The ultimate aim is see if rhizosphere microbiota are influenced by changes in root exudate composition resulting from abiotic stress. The abiotic variables we are focusing on at this stage are salinity, temperature and pH. This can be divided into two questions: (a) how do plant exudates change in response to abiotic stress, and (b) how do these changes influence bacteria. In order to test this we will produce plant exudates under controlled stressed conditions, measure their composition and measure bacterial growth in these exudates. Data has also been produced from synthetic community experiments comparing the community composition under a variety of controlled stress conditions (temperature, salinity, pH, and phosphate).
The work (proposal:https://doi.org/10.46936/10.25585/60000944) conducted by the U.S. Department of Energy Joint Genome Institute (https://ror.org/04xm1d337), a DOE Office of Science User Facility, is supported by the Office of Science of the U.S. Department of Energy operated under Contract No. DE-AC02-05CH11231.
Project description:We established a novel model to assess the function of proteins under in vivo conditions. The model relies on the expansion of HEK293 cells in immunodeficient NOD.Scid mice. To validate the novel model, we performed microarray gene expression profiling of NOD.Scid-expanded HEK293 cells relative to conventionally cultivated cells. Microarray analysis revealed that cell expansion in NOD.Scid mice restored an imbalanced chaperone system without inducing a major upregulation of the entire protein folding machinery. Human embryonic kidney (HEK293) cells were injected subcutaneously into immunodeficient NOD.Scid mice. After three weeks, the expanded cell pellet was isolated, and total RNA was extracted. In parallel, total RNA was prepared from HEK293 cells cultivated in vitro under standard cell culture conditions in a commercially available medium containing 450 mg/dl glucose (DMEM). Microarray gene expression profiling was performed to determine differential gene expression between in vivo expanded and in vitro cultivated HEK293 cells. Two biological replicates for each condition were made (in vitro cultivated HEK293 cells: Cells-1 and Cells-2; and NOD.Scid-expanded HEK293 cells: Scid-1 and Scid-2).
Project description:The resident skin microbiota plays a fundamental role in the control of skin physiology and growing evidence support the idea that, at this barrier site, both immunity and inflammatory processes are controlled by skin resident microbiota. However, how defined skin microbes influence the skin immune system under both steady state conditions and inflammatory settings remains poorly understood. Obesity has been linked to increased prevalence of skin inflammatory disorders. We used microarray analysis to investigate how Corynebacterium spp., dominant members of the skin microbiota of mice and humans, influence gene expression in the skin in mice under normal diet or a high-fat diet regimen.