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Reproducible and consistent quantification of the Saccharomyces cerevisiae proteome by SWATH-MS.


ABSTRACT: Data from ProteomeXchange, PXD ID: PXD001010. File: nselevse_L120203_020-Yeast OGE 11- 4 ul - IDA20-150ms.mgf. Published as part of Mol Cell Proteomics. 2015 Jan 5 . From the Abstract: {{i}} ... We sampled cell cultures in biological triplicates at six time points following the application of osmotic stress and acquired single injection DIA datasets on a high-resolution 5600 tripleTOF instrument operated in SWATH mode. Proteins were quantified by the targeted extraction and integration of transition signal groups from the SWATH-MS datasets for peptides that are proteotypic for specific yeast proteins ... {{/i}}

INSTRUMENT(S): Instrument

ORGANISM(S): Yeast

DISEASE(S): Not Available

SUBMITTER: Selevsek N, et al.  

PROVIDER: GPM32320007153 | GPMDB |

REPOSITORIES: GPMDB

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Reproducible and consistent quantification of the Saccharomyces cerevisiae proteome by SWATH-mass spectrometry.

Selevsek Nathalie N   Chang Ching-Yun CY   Gillet Ludovic C LC   Navarro Pedro P   Bernhardt Oliver M OM   Reiter Lukas L   Cheng Lin-Yang LY   Vitek Olga O   Aebersold Ruedi R  

Molecular & cellular proteomics : MCP 20150105 3


Targeted mass spectrometry by selected reaction monitoring (S/MRM) has proven to be a suitable technique for the consistent and reproducible quantification of proteins across multiple biological samples and a wide dynamic range. This performance profile is an important prerequisite for systems biology and biomedical research. However, the method is limited to the measurements of a few hundred peptides per LC-MS analysis. Recently, we introduced SWATH-MS, a combination of data independent acquisi  ...[more]

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