Matsumoto_Verification of MRM assays using in vitro proteome
Ontology highlight
ABSTRACT:
ORGANISM(S): Homo Sapiens (human)
SUBMITTER: Masaki Matsumoto
PROVIDER: PXD004902 | JPOST Repository | Sat Dec 03 00:00:00 GMT 2016
REPOSITORIES: jPOST
Dataset's files
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Publications
A large-scale targeted proteomics assay resource based on an in vitro human proteome.
Matsumoto Masaki M Matsuzaki Fumiko F Oshikawa Kiyotaka K Goshima Naoki N Mori Masatoshi M Kawamura Yoshifumi Y Ogawa Koji K Fukuda Eriko E Nakatsumi Hirokazu H Natsume Tohru T Fukui Kazuhiko K Horimoto Katsuhisa K Nagashima Takeshi T Funayama Ryo R Nakayama Keiko K Nakayama Keiichi I KI
Nature methods 20161226 3
Targeted proteomics approaches are of value for deep and accurate quantification of protein abundance. Extending such methods to quantify large numbers of proteins requires the construction of predefined targeted assays. We developed a targeted proteomics platform-in vitro proteome-assisted multiple reaction monitoring (MRM) for protein absolute quantification (iMPAQT)-by using >18,000 human recombinant proteins, thus enabling protein absolute quantification on a genome-wide scale. Our platform ...[more]
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