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RDA-seq (Next generation sequencing of Representational Difference Analysis products) of wheat lodicules

ABSTRACT: RNA from wheat lodicules in two highly-chasmogamous (HCH) (Piko and Poezja) and two low-chasmogamy (LCH) (Euforia and KWS Dacanto) varieties at two developmental stages - pre-flowering and early flowering were used to Representational Difference Analysis (cDNA-RDA). Lodicules at the pre-flowering were used as testers while the lodicules early flowering were selected as driver probes. This procedure results in accumulation of unique sequences upregulated in pre-flowering. The analysis was taken in three replicates for each variety (Piko, Poezja, Euforia, KWS Dacanto). The tester:driver ratio in three subsequent rounds of subtractive hybridization increased from 1:50, and 1:400 to 1:200000. Difference products obtained from the third subtraction in the size range from 200 to 800 bp were sequenced.

INSTRUMENT(S): Illumina MiSeq

ORGANISM(S): Triticum aestivum

SUBMITTER: Beata Bakera

PROVIDER: E-MTAB-12139 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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