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Dark and Bright sides of TMT tag for phosphoproteome analysis

ABSTRACT: The aim of this study is to figure out the mechanism of the loss of TMT labeling phosphopeptides during phosphopeptides purification by using TiO2 and IMAC and report the dark (cons) and bright (pros) sites of TMT tag for phosphoproteome analysis.

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Yasushi Ishihama

PROVIDER: PXD008506 | JPOST Repository | Thu Dec 20 00:00:00 GMT 2018

REPOSITORIES: jPOST

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			Action	DRS
	160219Fe_HT_1.raw	Raw
	160219Fe_HT_2.raw	Raw
	160219Fe_HT_3.raw	Raw
	160219Fe_LF_1.raw	Raw
	160219Fe_LF_2.raw	Raw

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JPST000370-4 Dark and Bright sides of TMT tag for phosphoproteome analysis [Reanalysis: JPST000370]

Project description:The aim of this study is to figure out the mechanism of the loss of TMT labeling phosphopeptides during phosphopeptides purification by using TiO2 and IMAC and report the dark (cons) and bright (pros) sites of TMT tag for phosphoproteome analysis. [Original project description]

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JPST000370-3 Dark and Bright sides of TMT tag for phosphoproteome analysis [Reanalysis: JPST000370]

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JPST000370-1 Dark and Bright sides of TMT tag for phosphoproteome analysis [Reanalysis: JPST000370]

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The bright and the dark sides of L-carnitine supplementation: a systematic review.

Project description:BACKGROUND:L-carnitine (LC) is used as a supplement by recreationally-active, competitive and highly trained athletes. This systematic review aims to evaluate the effect of prolonged LC supplementation on metabolism and metabolic modifications. METHODS:A literature search was conducted in the MEDLINE (via PubMed) and Web of Science databases from the inception up February 2020. Eligibility criteria included studies on healthy human subjects, treated for at least 12?weeks with LC administered orally, with no drugs or any other multi-ingredient supplements co-ingestion. RESULTS:The initial search retrieved 1024 articles, and a total of 11 studies were finally included after applying inclusion and exclusion criteria. All the selected studies were conducted with healthy human subjects, with supplemented dose ranging from 1?g to 4?g per day for either 12 or 24?weeks. LC supplementation, in combination with carbohydrates (CHO) effectively elevated total carnitine content in skeletal muscle. Twenty-four-weeks of LC supplementation did not affect muscle strength in healthy aged women, but significantly increased muscle mass, improved physical effort tolerance and cognitive function in centenarians. LC supplementation was also noted to induce an increase of fasting plasma trimethylamine-N-oxide (TMAO) levels, which was not associated with modification of determined inflammatory nor oxidative stress markers. CONCLUSION:Prolonged LC supplementation in specific conditions may affect physical performance. On the other hand, LC supplementation elevates fasting plasma TMAO, compound supposed to be pro-atherogenic. Therefore, additional studies focusing on long-term supplementation and its longitudinal effect on the cardiovascular system are needed.

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Loss of Bright/Arid3A reprograms MEFs to a pluripotent state

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Loss of Bright/Arid3A reprograms MEFs to a pluripotent state

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The bright and dark sides of social media usage during the COVID-19 pandemic: Survey evidence from Japan.

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