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JPST000370-2 Dark and Bright sides of TMT tag for phosphoproteome analysis [Reanalysis: JPST000370]

ABSTRACT: The aim of this study is to figure out the mechanism of the loss of TMT labeling phosphopeptides during phosphopeptides purification by using TiO2 and IMAC and report the dark (cons) and bright (pros) sites of TMT tag for phosphoproteome analysis. [Original project description]

ORGANISM(S): Epithelial Cell

SUBMITTER: Yasushi Ishihama

PROVIDER: RPXD034679 | JPOST Repository | Sat Mar 25 00:00:00 GMT 2023

REPOSITORIES: jPOST

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			Action	DRS
	160407Fe_Hela_IMAC_TMT_1.maxq.txt	Txt
	160407Fe_Hela_IMAC_TMT_1.txt	Txt
	160407Fe_Hela_IMAC_TMT_2.maxq.txt	Txt
	160407Fe_Hela_IMAC_TMT_2.txt	Txt
	160407Fe_Hela_IMAC_TMT_3.maxq.txt	Txt

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Dark and Bright sides of TMT tag for phosphoproteome analysis

Project description:The aim of this study is to figure out the mechanism of the loss of TMT labeling phosphopeptides during phosphopeptides purification by using TiO2 and IMAC and report the dark (cons) and bright (pros) sites of TMT tag for phosphoproteome analysis.

2018-12-20 | PXD008506 | JPOST Repository

JPST000370-1 Dark and Bright sides of TMT tag for phosphoproteome analysis [Reanalysis: JPST000370]

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JPST000370-4 Dark and Bright sides of TMT tag for phosphoproteome analysis [Reanalysis: JPST000370]

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JPST000370-3 Dark and Bright sides of TMT tag for phosphoproteome analysis [Reanalysis: JPST000370]

2023-03-25 | RPXD034680 | JPOST Repository

The bright and the dark sides of L-carnitine supplementation: a systematic review.

Project description:BACKGROUND:L-carnitine (LC) is used as a supplement by recreationally-active, competitive and highly trained athletes. This systematic review aims to evaluate the effect of prolonged LC supplementation on metabolism and metabolic modifications. METHODS:A literature search was conducted in the MEDLINE (via PubMed) and Web of Science databases from the inception up February 2020. Eligibility criteria included studies on healthy human subjects, treated for at least 12?weeks with LC administered orally, with no drugs or any other multi-ingredient supplements co-ingestion. RESULTS:The initial search retrieved 1024 articles, and a total of 11 studies were finally included after applying inclusion and exclusion criteria. All the selected studies were conducted with healthy human subjects, with supplemented dose ranging from 1?g to 4?g per day for either 12 or 24?weeks. LC supplementation, in combination with carbohydrates (CHO) effectively elevated total carnitine content in skeletal muscle. Twenty-four-weeks of LC supplementation did not affect muscle strength in healthy aged women, but significantly increased muscle mass, improved physical effort tolerance and cognitive function in centenarians. LC supplementation was also noted to induce an increase of fasting plasma trimethylamine-N-oxide (TMAO) levels, which was not associated with modification of determined inflammatory nor oxidative stress markers. CONCLUSION:Prolonged LC supplementation in specific conditions may affect physical performance. On the other hand, LC supplementation elevates fasting plasma TMAO, compound supposed to be pro-atherogenic. Therefore, additional studies focusing on long-term supplementation and its longitudinal effect on the cardiovascular system are needed.

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Loss of Bright/Arid3A reprograms MEFs to a pluripotent state

Project description:Bright null mouse embryonic stem cells and spontaneously reprogrammed Bright null cells compared to WT mouse embryonic stem cells;Work further described in Popowski et. al 2013 Bright null mouse embryonic stem cells and wildtype mouse embryonic stem cells differentiated in embryoid bodies at day 6 and day 15 Total RNA from each cell line using indicated replicates

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Comparison of whole transcript and 3’ RNA Sequencing methods

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Loss of Bright/Arid3A reprograms MEFs to a pluripotent state

2013-11-25 | GSE52692 | GEO

The bright and dark sides of social media usage during the COVID-19 pandemic: Survey evidence from Japan.

Project description:The spread of misinformation on the internet regarding the COVID-19 pandemic, such as unproven or fake cures, has been a serious concern. However, the extent to which social media usage affects individuals' health behavior, particularly when reliable information is scarce, is not well understood. This study evaluates the impact of social media usage on individuals' responses to the COVID-19 pandemic, such as demand for necessities and social distancing. We conduct an original online survey of 1804 Japanese respondents in March 2020. Japan is suitable because it confirmed COVID-19 cases earlier than most other countries. Scientific evidence about the coronavirus and protective measures was scarce in the initial pandemic phase, despite the spread of unconfirmed rumors. Our analysis focuses on the usage of Twitter, Facebook, and Instagram. We use the entropy balancing method to control for heterogeneity in observed characteristics between social media users and non-users. The results show that while users are more likely to maintain social distancing practices, they are also more likely to take measures whose reliability is not scientifically confirmed, such as eating fermented soybeans. Although previous studies emphasize the negative effects of social media, our results suggest that it has both bright and dark sides.

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