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JPST000370-2 Dark and Bright sides of TMT tag for phosphoproteome analysis [Reanalysis: JPST000370]

ABSTRACT: The aim of this study is to figure out the mechanism of the loss of TMT labeling phosphopeptides during phosphopeptides purification by using TiO2 and IMAC and report the dark (cons) and bright (pros) sites of TMT tag for phosphoproteome analysis. [Original project description]

ORGANISM(S): Epithelial Cell

SUBMITTER: Yasushi Ishihama

PROVIDER: RPXD034679 | JPOST Repository | Sat Mar 25 00:00:00 GMT 2023

REPOSITORIES: jPOST

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Dark and Bright sides of TMT tag for phosphoproteome analysis

Project description:The aim of this study is to figure out the mechanism of the loss of TMT labeling phosphopeptides during phosphopeptides purification by using TiO2 and IMAC and report the dark (cons) and bright (pros) sites of TMT tag for phosphoproteome analysis.

2018-12-20 | PXD008506 | JPOST Repository

JPST000370-3 Dark and Bright sides of TMT tag for phosphoproteome analysis [Reanalysis: JPST000370]

2023-03-25 | RPXD034680 | JPOST Repository

JPST000370-4 Dark and Bright sides of TMT tag for phosphoproteome analysis [Reanalysis: JPST000370]

2023-03-25 | RPXD034681 | JPOST Repository

JPST000370-1 Dark and Bright sides of TMT tag for phosphoproteome analysis [Reanalysis: JPST000370]

2023-03-25 | RPXD034678 | JPOST Repository

The bright and the dark sides of L-carnitine supplementation: a systematic review.

Project description:BACKGROUND:L-carnitine (LC) is used as a supplement by recreationally-active, competitive and highly trained athletes. This systematic review aims to evaluate the effect of prolonged LC supplementation on metabolism and metabolic modifications. METHODS:A literature search was conducted in the MEDLINE (via PubMed) and Web of Science databases from the inception up February 2020. Eligibility criteria included studies on healthy human subjects, treated for at least 12?weeks with LC administered orally, with no drugs or any other multi-ingredient supplements co-ingestion. RESULTS:The initial search retrieved 1024 articles, and a total of 11 studies were finally included after applying inclusion and exclusion criteria. All the selected studies were conducted with healthy human subjects, with supplemented dose ranging from 1?g to 4?g per day for either 12 or 24?weeks. LC supplementation, in combination with carbohydrates (CHO) effectively elevated total carnitine content in skeletal muscle. Twenty-four-weeks of LC supplementation did not affect muscle strength in healthy aged women, but significantly increased muscle mass, improved physical effort tolerance and cognitive function in centenarians. LC supplementation was also noted to induce an increase of fasting plasma trimethylamine-N-oxide (TMAO) levels, which was not associated with modification of determined inflammatory nor oxidative stress markers. CONCLUSION:Prolonged LC supplementation in specific conditions may affect physical performance. On the other hand, LC supplementation elevates fasting plasma TMAO, compound supposed to be pro-atherogenic. Therefore, additional studies focusing on long-term supplementation and its longitudinal effect on the cardiovascular system are needed.

| S-EPMC7507632 | biostudies-literature

Loss of Bright/Arid3A reprograms MEFs to a pluripotent state

Project description:Bright null mouse embryonic stem cells and spontaneously reprogrammed Bright null cells compared to WT mouse embryonic stem cells;Work further described in Popowski et. al 2013 Bright null mouse embryonic stem cells and wildtype mouse embryonic stem cells differentiated in embryoid bodies at day 6 and day 15 Total RNA from each cell line using indicated replicates

2013-11-25 | E-GEOD-52692 | biostudies-arrayexpress

Comparison of whole transcript and 3’ RNA Sequencing methods

Project description:We used two RNA-Seq methods to measure the the global transcription levels in mouse liver cells. The data here provide insight into the pros and cons of whole transcript method and 3' RNA-Seq method.

2018-10-24 | GSE116949 | GEO

Loss of Bright/Arid3A reprograms MEFs to a pluripotent state

2013-11-25 | GSE52692 | GEO

The bright and dark sides of protein conformational switches and the unifying forces of infections.

Project description:It is now established that a protein can switch between multiple conformations to enable altered functions. Several pathogens including SARS COV2 utilize context-dependent conformational switches of particular proteins to invade host membrane to establish infections. In this perspective, we first discuss the understanding of the conformational switch of a protein towards the productive infections as a dark side of nature. Next, the unexplored binary combination of the sequences of SARS COV2 spike protein and the similarity with diverse pathogen derived proteins have been discussed to obtain novel molecular insights into the process of infection.

| S-EPMC7363679 | biostudies-literature

Characterizing the tumor immune microenvironment of ependymomas using targeted gene expression profiles and RNA sequencing

Project description:Defining the tumor immune microenvironment (TIME) of patients using transcriptome analysis is gaining more popularity. Here, we examined and discussed the pros and cons of using RNA -sequencing for Fresh Frozen (FF) samples and targeted gene expression immune profiles (NanoString) for Formalin-Fixed, Paraffin-Embedded (FFPE) samples to characterize the TIME of ependymoma samples.

2022-10-25 | GSE216478 | GEO

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