Dataset Information

E.coli and Human proteome

ABSTRACT: To measure quantification accuracy, we generated two proteome samples of E.coli and human cell lysate. E.coli tryptic peptides were tagged with four different labels of reporter ions (m/z: 114, 115, 116 and 117). These labeled peptides were mixed at the following ratios: 114:115 = 10:1, 114:117 = 5:1, 114:116 and 117:115 = 2:1, and 116:117 = 2.5:1. Human tryptic peptides were tagged with two different labels of reporter ions at 114 and 115, and these samples were mixed 1:1.

ORGANISM(S): Homo Sapiens (human) Escherichia Coli

SUBMITTER: Mio Iwasaki

PROVIDER: PXD011913 | JPOST Repository | Tue May 14 00:00:00 BST 2019

REPOSITORIES: jPOST

ACCESS DATA

Dataset's files

Source:

			Action	DRS
	170817mi-09-Ecoli-DH5a-Mix-GL7-CiRA1-DA-IS5L2.wiff	Wiff
	170817mi-09-Ecoli-DH5a-Mix-GL7-CiRA1-DA-IS5L2.wiff.scan	Wiff
	170817mi-12-Ecoli-DH5a-HeLa-Mix-GL7-CiRA1-DA-IS5L2.wiff	Wiff
	170818-07-Ecoli-DH5a-HeLa-Mix-GL8-CiRA2-DA-IS5L2.wiff.scan	Wiff
	170818-10-Ecoli-DH5a-Mix-GL8-CiRA2-DA-IS5L2.wiff	Wiff

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Publications

Removal of Interference MS/MS Spectra for Accurate Quantification in Isobaric Tag-Based Proteomics.

Iwasaki Mio M Tabata Tsuyoshi T Kawahara Yuka Y Ishihama Yasushi Y Nakagawa Masato M

Journal of proteome research 20190506 6

Rapid progress in mass spectrometry (MS) has made comprehensive analyses of the proteome possible, but accurate quantification remains challenging. Isobaric tags for relative and absolute quantification (iTRAQ) is widely used as a tool to quantify proteins expressed in different cell types and various cellular conditions. The quantification precision of iTRAQ is quite high, but the accuracy dramatically decreases in the presence of interference peptides that are coeluted and coisolated with the ...[more]

PMID: 31039306

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