Dataset Information

Proteomics analysis on lipid metabolism in Elaeis guineensis and Elaeis oleifera

ABSTRACT: Total proteins were extracted from randomly selected mesocarp fruitlets and subjected to label-free liquid chromatography mass spectrometry. Mass spectra were acquired using Thermo Xcalibur and deconvoluted with Proteome Discoverer 2.2 to create the mass list. Sequsest search engine was used to match the generated mass list against Elaeis guineensis and Phoenix datylifera protein sequences in NCBI. Standard protein search parameters were applied.

ORGANISM(S): Elaeis Guineensis

SUBMITTER: Benjamin Lau Yii Chung

PROVIDER: PXD017436 | JPOST Repository | Mon Jun 01 00:00:00 BST 2020

REPOSITORIES: jPOST

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Proteomics analysis on lipid metabolism in Elaeis guineensis and Elaeis oleifera.

Lau Benjamin Yii Chung BYC Amiruddin Mohd Din MD Othman Abrizah A

Data in brief 20200516

Proteome data was obtained from the fruit mesocarps of the two oil palm species, namely, the African Elaeis guineensis (commercial tenera or commonly known as D x P and MPOB-Nigerian tenera) and the South American Elaeis oleifera. Total proteins were extracted from randomly selected fruitlets and subjected to proteomics characterisation by means of liquid chromatography mass spectrometry. Number of proteins identified, the grouping of the biological replicates from five development ...[more]

PMID: 32462070

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Project description:A critical step during fertilization is the sperm acrosome reaction in which the acrosome releases its contents allowing the spermatozoa to penetrate the egg investments. The sperm acrosomal contents are composed of both soluble material and an insoluble material called the acrosomal matrix (AM). The AM is thought to provide a stable structure from which associated proteins are differentially released during fertilization. Mouse acrosomal matrices were isolated from 2 different populations of epididymal spermatozoa: AM_caput from caput epididymal spermatozoa (2 replicates) and AM_cauda from cauda epididymal spermatozoa (3 replicates). Proteins were separated by SDS-PAGE on a 15% polyacrylamide gel. After electrophoresis, proteins were stained with Coomassie blue. Gel lanes were cut into 8–9 slices and each slice placed into a 0.5 ml microcentrifuge tube. The gel pieces were washed to destain the gels. Reduction was performed with dithiothreitol solution. After reduction, the proteins were alkylated. The gel pieces were washed, dehydrated and air-dried. The digestion was proceed with trypsin and peptides extracted. The peptides obtained from in-gel digestion were analyzed by nano-flow nano-LC-MS/MS using an LTQ-XL ion trap mass spectrometer. To get the maximum number of publicly available sequences for analyses, the mouse sequences from Ensembl were merged with those from NCBI. The database was built using BioPerl modules. Specifically, from Ensembl two files were downloaded and merged including Mus_musculus.NCBIM37.64.pep.all.fa.gz (containing the superset of all translations resulting from Ensembl known or novel gene predictions) and Mus_musculus.NCBIM37.64.pep.abinitio.fa.gz (containing translations resulting from “ab initio” gene prediction algorithms such as SNAP and GENSCAN). From NCBI, the nonredundant (nr) file (on 11/2011) was downloaded and mouse sequences extracted (Taxonomy ID = 10090). This file was merged with the Ensembl sequences to create a file containing 356,881 sequences of which some were redundant. Duplicates were removed based on identical amino acid sequences to get 203,220 unique sequences used to build our blast database. Spectra obtained from the trypsin digestion products using the LTQ Orbitrap XL mass spectrometer were identified by the Proteome Discoverer (version 1.3) program, based on SEQUEST cluster as a search engine (University of Washington, licensed to Thermo Electron Corp., San Jose, CA) against our mouse database (203,220 nonredundant protein sequences). The search engine used the following parameters: precursor ion mass tolerance, 2.5 Da; fragment ion mass tolerance, 0.8 Da; fully tryptic enzyme specificity; two missed cleavages; dynamic modifications of cysteine carbamidomethylation and of methionine oxidation. The proportion of false positive assignations among the tentative peptide identifications, also called false discovery rate (FDR), has been estimated by using decoy databases constructed from the target database and was set at 1%.

			Action	DRS
	EGHOLOvsEO.mzid	Mzid
	EGHO_onlybiorep.msf	Msf
	EGLO_onlybiorep.msf	Msf
	EO_onlybiorep.msf	Msf

Dataset Information

Proteomics analysis on lipid metabolism in Elaeis guineensis and Elaeis oleifera

Dataset's files

Publications

Proteomics analysis on lipid metabolism in <i>Elaeis guineensis</i> and <i>Elaeis oleifera</i>.

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