Ontology highlight
ABSTRACT:
INSTRUMENT(S): instrument model, LCQ Deca XP Plus
ORGANISM(S): Gallus Gallus (chicken)
TISSUE(S): Bursa Of Fabricius
SUBMITTER: DAVE THORNEYCROFT
PROVIDER: PRD000755 | Pride | 2005-08-21
REPOSITORIES: Pride
Items per page: 1 - 5 of 12 |
Journal of proteome research 20050301 2
Differential detergent fractionation (DDF), which relies on detergents to sequentially extract proteins from eukaryotic cells, has been used to increase proteome coverage of 2D-PAGE. Here, we used DDF extraction in conjunction with the nonelectrophoretic proteomics method of liquid chromatography and electrospray ionization tandem mass spectrometry. We demonstrate that DDF can be used with 2D-LC ESI MS2 for comprehensive cellular proteomics, including a large proportion of membrane proteins. Com ...[more]