Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Proteome of rice plasma membrane in flower

ABSTRACT: Proteins extracted from rice plasma membrane in flowers were identified with Orbitrap mass spectrometer.

ORGANISM(S): Oryza Sativa

SUBMITTER: Yukimoto Iwasaki

PROVIDER: PXD018199 | JPOST Repository | Fri Mar 26 00:00:00 GMT 2021

REPOSITORIES: jPOST

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	201907300.mgf	Mgf
	201907300.mzTab	Mztab
	2019073001.raw	Raw
	2019073002.raw	Raw
	2019073003.raw	Raw

Items per page:

1 - 5 of 20

Similar Datasets

Proteome of rice plasma membrane in root

Project description:Proteins extracted from rice plasma membrane in roots were identified with Orbitrap mass spectrometer.

2021-03-26 | PXD018201 | JPOST Repository

Proteome of rice plasma membrane in greening leaf

Project description:Proteins extracted from rice plasma membrane in greening leaves were identified with Orbitrap mass spectrometer.

2021-03-26 | PXD018200 | JPOST Repository

Proteome of rice plasma membrane in leaf sheath

Project description:Proteins extracted from rice plasma membrane in leaf sheaths were identified with Orbitrap mass spectrometer.

2021-03-26 | PXD018198 | JPOST Repository

Proteome of rice etiolated seedling

Project description:Proteins extracted from rice plasma membrane in etiolated seedlings were identified with Orbitrap mass spectrometer.

2021-03-26 | PXD018197 | JPOST Repository

Plasma Membrane Integrity Signaling

Project description:Plasma Membrane Integrity Signaling

| PRJNA701242 | ENA

The plasma membrane NADPH oxidase OsRbohA plays a crucial role in developmental regulation and drought-stress response in rice

2015-12-31 | GSE54466 | GEO

Plasma Membrane Integrity Signaling

Project description:To understand the transcriptional response of mammalian cells to plasma membrane stress we induced partial loss of plasmid membrane integrity by chemogenetic induction of pore forming proteins (Gasderimin and MLKL) or treatment with the detergent digitonin. We monitored transcription with mRNAseq in the immediate hours after plasma membrane damage and identified conserved transcriptional programs.

2021-10-04 | GSE166538 | GEO

The plasma membrane NADPH oxidase OsRbohA plays a crucial role in developmental regulation and drought-stress response in rice

Project description:Plasma membrane NADPH oxidases (NOXs) are major producers of reactive oxygen species (ROS) in plant cells under normal growth and stress conditions. Rice NOXs have multiple homologs but their functional mechanisms are largely unknown. We used microarrays to detail the global gene expression profiles in rice wild-type (WT, Dongjin) and a mutant osnox2 which loss the functions of OsNOX2 protein under drought and identified distinct classes of genes between the two type rice plants under both normal growth and drought stressed conditions. The youngest fully expanded leaves from 2.5-month-old WT and osnox2 plants (three replicates each), grown under normal growth (soil moisture, 47.3%) and drought conditions (soil moisture, 8.5%), were used for RNA extraction and hybridization on Affymetrix microarrays. Control: normal growth condition; Drought: drought stress condition.

2015-12-31 | E-GEOD-54466 | biostudies-arrayexpress

Comparative proteomic analysis of plasma membrane proteins in rice leaves reveals a vesicle trafficking network in plant immunity provoked by blast fungi

Project description:This project aims to analyze rice plasma membrane proteins related to resistance against rice blast infection. We extracted rice plasma membrane proteins before and after M.oryzae infection for 24h, and then used trypsin to digest and iTRAQ to label the peptides, HPLC-MS/MS was used to seperate and identify peptides. 1.1/0.909 fold change with p-value < 0.05 was used as threshold for differentially expressed proteins. 2,977 proteins were identified and 951 of which were found to be responsive to resistance against M. oryzae. Gene Ontology (GO) enrichment analysis, Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis and protein interaction network showed that plenty of proteins were involved in vesicle trafficking with obvious functional tendencies towards transport, vesicle-mediated transport, secretion, endocytosis and phagosome. 10 DEPs were validated at transcript level, and a SNARE protein named NPSN (novel plant-specific SNARE)13 actively responded to M. oryzae infection, and it contributed to rice blast resistance and mainly located at PM.

2022-05-19 | PXD028545 | Pride

Cytosolic and plasma membrane proteome analysis of rice leaves in response to MSP1 treatment

Project description:MSP1 is a Magnaporthe oryzae secreted protein that elicits defense responses in rice. However, the molecular mechanism of MSP1 action is largely elusive. Here, we employed a TMT-based quantitative proteomic analysis of cytoplasmic as well as plasma membrane proteins to decipher the MSP1 induced signalling in rice. This approach led to the identification of 6691 proteins of which 3049 were identified in the plasma membrane (PM) while 3642 were identified in the cytoplasmic fraction. A parallel phosphoproteome analysis led to the identification of 1906 phosphopeptides and integration of proteome and phosphoproteome data showed activation of proteins related to the proteolysis, jasmonic acid biosynthesis, redox metabolism and MAP kinase signaling pathways in response to MSP1 treatment. Further, MSP1 induced phosphorylation of some of the key proteins including RBOHB, MEKK1, MPK3/6, CDPK and CaM suggest activation of PAMP-triggered immunity (PTI) in response to MSP1 treatment. In essence, our results further support the functioning of MSP1 as a PAMP and provide an overview of the MSP1 induced signaling in rice leaves.

2019-08-30 | PXD014758 | Pride

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data