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Identification of pomalidomide-dependent CRBN binding proteins

ABSTRACT: The CRL4-FH-CRBN complex was purified from 293T cells stably expressing FH-CRBN and stained with CBB following SDS-PAGE. The purified complex was incubated with various cell extracts for 24 h in the presence or absence of pomalidomide. Bound proteins were then affinity-purified using anti-HA beads and subjected to SDS PAGE and CBB staining. Gel slices were prepared and subjected to MS.

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Takumi Ito and Hiroshi Handa

PROVIDER: PXD023928 | JPOST Repository | Wed Feb 02 00:00:00 GMT 2022

REPOSITORIES: jPOST

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			Action	DRS
	sample%201%20vs%20sample%202.xls	Xls
	sample%201.raw	Raw
	sample%202.raw	Raw
	sample%203%20vs%20sample%204.xls	Xls
	sample%203.raw	Raw

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