Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

In vivo phosphorylation of TOC1

ABSTRACT: Myc-tagged N-terminal portion of TOC1 or TOC1 mutant (S194A, S201A, T204A) were expressed in tobacco leaves and then immunoprecipitated. The immunoprecipitated samples were separated on SDS-PAGE gels, and then phosphorylation sites of TOC1 were identified from gel bands.

ORGANISM(S): Arabidopsis Thaliana (mouse-ear Cress)

SUBMITTER: Hirofumi Nakagami

PROVIDER: PXD026261 | JPOST Repository | Mon Oct 04 00:00:00 BST 2021

REPOSITORIES: jPOST

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	+PSNFL-1.RAW	Raw
	+PSNFL-2.RAW	Raw
	-PSNFL-1.RAW	Raw
	-PSNFL-2.RAW	Raw
	120120_Korea_GelSample_Myc-TOC1NT.sf3	Other

Items per page:

1 - 5 of 12

Similar Datasets

PrimPol-phosphoproteomics by IP-GeLC-MS/MS

Project description:Myc-tagged PrimPol expressed in HEK-293T cells was immunoprecipitated and separated by SDS-PAGE. Protein bands were excised and subjected to in-gel tryptic digestion and phosphopeptide enrichment by Fe-IMAC.

2023-08-22 | PXD043419 | JPOST Repository

Identification of FBP1 binding proteins in wild-type, G164D and F194S comparisons by LC-MS/MS analysis

Project description:Myc-tagged FBP1-WT, Myc-tagged FBP1-G164D, Myc-tagged FBP1-F194S or empty vector were transfected into FBP1-KO HepG2 cells. FBP1 complexes were immunoprecipitated with anti-Myc beads and then separated by SDS‒PAGE. Bands were removed from the gel, and trypsin-digested samples were subjected to liquid chromatography-tandem mass spectrometry (LC-MS/MS) using an Orbitrap Velos Pro (Thermo Scientific). MS/MS dataset analysis was performed using the Mascot software program (Matrix Science).

2023-05-20 | PXD042364 | JPOST Repository

Functional and proteomic insights into Aculeata venoms

Project description:Shotgun proteomics of whole venom and excised bands from SDS-PAGE gels

2023-03-02 | MSV000091399 | MassIVE

Identifying RasGAP SH2 binding partners in Drosophila using mass spectrometry

Project description:Drosophila S2 cells were transfected with RasGAP(WT), RasGAP(SH2*32*) and GFP control. 4 mg of S2 cell lysate was used to pulldown LAP and GFP tagged constructs using GFP-Trap. The pulled down fraction was loaded on SDS-PAGE gels and coomassie stained before several bands (a-u) from two independent but parallel experiments were excised and subjected to MS analysis.

2014-05-20 | PXD000176 | Pride

TATDN2 Resolution of R-Loops is Required for Survival of BRCA1-Mutant Cancer Cells

Project description:Immunoprecipitated protein using Myc-tagged TATDN2 from U2OS.

2023-06-16 | MSV000092192 | MassIVE

Archael ubiquitin-like SAMP3 is isopeptide-linked to Proteins by an UbaA-dependent mechanism

Project description:FLAG-SAMP3ylated proteins were immunoprecipitated from Haloferax volcanii using anti-FLAG affinity agarose and separated using SDS-PAGE. The Coomassie-stained IP-bands represented FLAG-SAMP3ylated proteins harvested from the wild type and also from the ubaA mutant as negative control. The Coomassie-lanes from the IP samples were cut into 10 fractions and in-gel tryptic digested and the tryptic peptides measured using Orbitrap Velos LC-MS/MS.

2020-07-02 | PXD000202 | Pride

neonatal rat cardiomyocytes LC-MS/MS

Project description:NRCMs were treated with ISO (10 μM) in the presence or absence of FGF18 (50 ng/ml) for 48 h. Protein bands at ~60 kDa were excised from the SDS-PAGE gels for LC-MS/MS analysis

2023-03-11 | PXD039587 | Pride

CC2_Navarre_14028srpoSlowhns-_IP_pHNStagged_antiHA

Project description:array analysis of immunoprecipitated DNA from tagged HNS proteins in 14028s mutant strain (rpoS low, hns minus) Keywords: ChIP-chip

2006-05-24 | GSE4881 | GEO

Phos-tag SDS-PAGE followed by LC-MS/MS analysis of in vitro phosphorylated Nab2, Foxk1, and HURP

Project description:Purified GST-fused Nab2, Foxk1, and HURP were incubated with ATP in the presence or absence of His-tagged active ERK in vitro, separated by Phos-tag SDS-PAGE, and visualized by CBB staining. The most shifted regions of protein bands on the Phos-tag-containing gels were excised, digested with trypsin, and analyzed by LC-MS/MS.

2022-03-03 | PXD028837 | JPOST Repository

In vivo and in vitro Genome wide binding (ChIP-Seq) of Bapx1 and Sox9

Project description:Bapx1 was endogenously tagged with S-Peptide and dissected vertebral columns from these tagged embryos at E12.5 were subjected to immunoprecipitation by S-peptide antibody. Simultaneously V5 tagged Bapx1 was also over expressed in NIH_3T3 cells and immunoprecipitated with V5 antibody. Concurrently vertebral column fro E12.5 mouse embryos were subjected to immunoprecipitation with Sox9 antobody to compare binding sites of Sox9 and BApx1 in the vertebral column of developing mouse embryo

2015-03-19 | GSE36672 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data