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Differences of proteome between c-MYC and L-MYC during cellular reprogramming

ABSTRACT: MYC proteins (c-MYC, n-MYC, and L-MYC) are essential for improving reprogramming efficiency. L-MYC can generate iPSC colonies more efficiently than c-MYC. Still, it remains unknown what is the functional differences between c-MYC and L-MYC during cellular reprogramming. In this study, we performed proteome analysis to tackle this question and found MYC Box 0 (MB0) and 2 (MB2), one of the functional domains conserved in MYC family protein, might cause the phenotypic differences between the MYC proteins.

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Mio Iwasaki

PROVIDER: PXD027079 | JPOST Repository | Sat Jan 08 00:00:00 GMT 2022

REPOSITORIES: jPOST

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			Action	DRS
	181004mi-02-Akifuji-SeV-Day3-CiRA1-GL9-DA.wiff	Wiff
	181004mi-02-Akifuji-SeV-Day3-CiRA1-GL9-DA.wiff.scan	Wiff
	181004mi-05-Akifuji-SeV-Day3-CiRA1-GL9-DA.wiff	Wiff
	181004mi-05-Akifuji-SeV-Day3-CiRA1-GL9-DA.wiff.scan	Wiff
	181004mi-08-Akifuji-SeV-Day3-CiRA1-GL9-DA.wiff	Wiff

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Publications

MYCL promotes iPSC-like colony formation via MYC Box 0 and 2 domains.

Akifuji Chiaki C Iwasaki Mio M Kawahara Yuka Y Sakurai Chiho C Cheng Yu-Shen YS Imai Takahiko T Nakagawa Masato M

Scientific reports 20211220 1

Human induced pluripotent stem cells (hiPSCs) can differentiate into cells of the three germ layers and are promising cell sources for regenerative medicine therapies. However, current protocols generate hiPSCs with low efficiency, and the generated iPSCs have variable differentiation capacity among different clones. Our previous study reported that MYC proteins (c-MYC and MYCL) are essential for reprogramming and germline transmission but that MYCL can generate hiPSC colonies more efficiently t ...[more]

PMID: 34930932

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Differences of phosphoproteome between c-MYC and L-MYC during cellular reprogramming

Project description:MYC proteins (c-MYC, n-MYC, and L-MYC) are essential for improving reprogramming efficiency. L-MYC can generate iPSC colonies more efficiently than c-MYC. Still, it remains unknown what is the functional differences between c-MYC and L-MYC during cellular reprogramming. In this study, we performed proteome analysis to tackle this question and found MYC Box 0 (MB0) and 2 (MB2), one of the functional domains conserved in MYC family protein, might cause the phenotypic differences between the MYC proteins.

2022-01-08 | PXD027078 | JPOST Repository

Differences in binding partners between c-MYC and L-MYC during cellular reprogramming

2022-01-07 | PXD027083 | JPOST Repository

Differences in protein domains between c-MYC and L-MYC during cellular reprogramming

2022-01-07 | PXD027086 | JPOST Repository

Myc and SAGA Rewire an Alternative Splicing Network During Early Somatic Cell Reprogramming [Reprogramming_ChIPSEQ]

Project description:Embryonic stem cells are maintained in a self-renewing and pluripotent state by multiple regulatory pathways. Pluripotent-specific transcriptional networks are sequentially reactivated as somatic cells reprogram to achieve pluripotency. How epigenetic regulators modulate this process and contribute to somatic cell reprogramming is not clear. Here we perform a functional RNAi screen to identify the earliest epigenetic regulators required for reprogramming. We identify components of the SAGA histone acetyltransferase complex, in particular Gcn5, as critical regulators of reprogramming initiation. Furthermore, we show in mouse pluripotent stem cells that Gcn5 strongly associates with Myc and that upon initiation of somatic reprogramming, Gcn5 and Myc form a positive feed forward loop that activates a distinct alternative splicing network and the early acquisition of pluripotency-associated splicing events. These studies expose a Myc-SAGA pathway that drives expression of an essential alternative splicing regulatory network during somatic cell reprogramming. Examination of Myc-chromatin interactions in reprogramming cells

2015-04-15 | E-GEOD-67694 | biostudies-arrayexpress

Finding functional differences between species in a microbial community

Project description:Finding functional differences between species in a microbial community

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A helicase-independent role of DHX15 promotes MYC stability and acute leukemia cell survival

Project description:DHX15 has been implicated in RNA splicing and ribosome biogenesis, primarily functioning as an RNA helicase. To systematically assess the cellular role of DHX15, we conducted proteomic analysis to investigate the landscape of DHX15 interactome, and identified MYC as a binding partner. DHX15 co-localizes with MYC in cells and directly interacts with MYC in vitro. Importantly, DHX15 contributes to MYC protein stability at the post-translational level and independent of its RNA binding capacity. Mechanistic investigation reveals that DHX15 interferes the interaction between MYC and FBXW7, thereby preventing MYC polyubiquitylation and proteasomal degradation. Consequently, abrogation of DHX15 drastically inhibits MYC-mediated transcriptional output. While DHX15 depletion blocks T-cell development and leukemia cell survival as we recently reported, overexpression of MYC significantly rescues the phenotypic defects. These findings shed new light on the essential role of DHX15 in mammalian cells and suggest that maintaining sufficient MYC expression is a significant contributor to DHX15-mediated cellular functions.

2024-01-26 | PXD046692 | Pride

Myc and SAGA Rewire an Alternative Splicing Network During Early Somatic Cell Reprogramming [mESCs_ChIPSEQ]

2015-04-15 | E-GEOD-67710 | biostudies-arrayexpress

Myc and SAGA Rewire an Alternative Splicing Network During Early Somatic Cell Reprogramming [Reprogramming_RNASEQ]

2015-04-15 | E-GEOD-67715 | biostudies-arrayexpress

Functional conservation of jasmonate-related MYC transcription factors in Marchantia polymorpha

Project description:The lipid-derived phytohormone jasmonoyl-isoleucine (JA-Ile) regulates plant immunity, growth and development in vascular plants by activating genome-wide transcriptional reprogramming. In Arabidopsis, this is largely orchestrated by the master regulator MYC2 and related transcription factors (TFs). However, the TFs activating this pathway in basal plant lineages are currently unknown. We report the functional conservation of MYC-related TFs between the eudicot Arabidopsis thaliana and the liverwort Marchantia polymorpha, a plant belonging to one of the most basal land-plants lineages. Phylogenetic analysis suggests that MYC function first appeared in charophycean algae, and therefore predates the evolutionary appearance of any other jasmonate pathway component. Marchantia possesses two functionally interchangeable MYC genes, one in females and one in males. Similar to AtMYC2, MpMYCs showed nuclear localization, interaction with JAZ-repressors, and regulation by light. Phenotypic and molecular characterization of loss- or gain-of-function mutants demonstrated that MpMYCs are necessary and sufficient for the activation of the pathway in Marchantia, but unlike their Arabidopsis orthologs, do not regulate fertility. Our results show that despite 450 million years of independent evolution, MYCs are functionally conserved between bryophytes and eudicots. Genetic conservation in one of the most basal lineages suggests that MYC function existed in the common ancestor of land plants and evolved from a pre-existing MYC function in charophycean algae.

2019-03-19 | GSE124159 | GEO

Burkitt Lymphoma beyond MYC translocation:N-MYC and DNA methyl transferases dysregulation

Project description:Identification of miRNA differentially expressed between MYC-translocation positive and and MYC-translocation negative Burkitt Lymphoma and their impact on gene expression profile Study of microRNA profile of MYC-translocation positive and MYC-translocation negative Burkitt Lymphoma cases in order to uncover possible differences at the molecular level

2015-08-05 | E-GEOD-71471 | biostudies-arrayexpress

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