Dataset Information

Mpe1 senses the polyadenylation signal in pre-mRNA to promote efficient cleavage and control polyadenylation

ABSTRACT: Crosslinking mass spectrometry dataset of the Mpe1 and Cft2 bound to the yeast CPF polymerase module. Sulfo-SDA is the crosslinker used.

ORGANISM(S): Saccharomyces Cerevisiae (baker's Yeast)

SUBMITTER: Juri Rappsilber

PROVIDER: PXD027482 | JPOST Repository | Thu Jan 12 00:00:00 GMT 2023

REPOSITORIES: jPOST

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Dataset's files

Source:

			Action	DRS
	B210320_15_Lumos_FO_CO_FO_Passmore_PAM1-CFT2-Mpe1-RNA_SDA_pepSEC5_rep1.raw	Raw
	B210320_16_Lumos_FO_CO_FO_Passmore_PAM1-CFT2-Mpe1-RNA_SDA_pepSEC5_rep2.raw	Raw
	B210320_17_Lumos_FO_CO_FO_Passmore_PAM1-CFT2-Mpe1-RNA_SDA_pepSEC6_rep1.raw	Raw
	B210320_18_Lumos_FO_CO_FO_Passmore_PAM1-CFT2-Mpe1-RNA_SDA_pepSEC6_rep2.raw	Raw
	B210320_19_Lumos_FO_CO_FO_Passmore_PAM1-CFT2-Mpe1-RNA_SDA_pepSEC7_rep1.raw	Raw

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Publications

Mpe1 senses the binding of pre-mRNA and controls 3' end processing by CPF.

Rodríguez-Molina Juan B JB O'Reilly Francis J FJ Fagarasan Holly H Sheekey Eleanor E Maslen Sarah S Skehel J Mark JM Rappsilber Juri J Passmore Lori A LA

Molecular cell 20220517 13

Most eukaryotic messenger RNAs (mRNAs) are processed at their 3' end by the cleavage and polyadenylation specificity factor (CPF/CPSF). CPF mediates the endonucleolytic cleavage of the pre-mRNA and addition of a polyadenosine (poly(A)) tail, which together define the 3' end of the mature transcript. The activation of CPF is highly regulated to maintain the fidelity of RNA processing. Here, using cryo-EM of yeast CPF, we show that the Mpe1 subunit directly contacts the polyadenylation signal sequ ...[more]

PMID: 35584695

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