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Phosphorylation of CAD1, PLDdelta, NDT1, RPM1 proteins induced resistance in tomatoes infected by Ralstonia solanacearum

ABSTRACT: Ralstonia solanacaerum is one of the most devastating bacteria causing bacterial wilt disease in more than 200 species of plants, especially family Solanaceae. To cope with this, plants evolved different resistance mechanisms depending on the signal transduction after the perception. Phosphorylation is the central regulation of the signal transduction pathway. As the earliest signaling events are activated within minutes, we investigated comparative phosphoproteomics analysis of the stems of resistant and susceptible tomatoes at 15 min, 30 min, 24 h, and 48 h after inoculation by Ralstonia solanacearum to find out the phosphorylated proteins involved in the induced resistance.

ORGANISM(S): Cellular Organisms

SUBMITTER: Sittiruk Roytrakul

PROVIDER: PXD030242 | JPOST Repository | Thu Dec 09 00:00:00 GMT 2021

REPOSITORIES: jPOST

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Project description:Grapevine downy mildew caused by the Oomycete Plasmopara viticola is one of the most important diseases affecting Vitis spp. However, all cultivated European grapevine varieties are susceptible to P. viticola and the resistance needs to be introduced from other Vitaceae. Segregating populations derived from Muscadinia rotundifolia, a species closely related to Vitis, lead to the identification and mapping of two resistance genes, named Rpv1 and Rpv2. The macroscopic phenotypes of the resistance mediated by the two loci are different. Rpv2 plants completely inhibit P. viticola sporulation and produce very small necrotic lesions. In contrast, Rpv1 plants allow a rather limited but visible sporulation of P. viticola. The aim of the study is to understand the gene expression changes associated with downy mildew resistance mediated by these two loci. Gene expression patterns after P. viticola inoculation or mock inoculation are compared in incompatible (resistant plants bearing Rpv1 or Rpv2 locus) and compatible (susceptible plants with no resistance loci) interactions, using the Vitis vinifera GeneChip from Affymetrix. In order to limit the effect of the genetic background, the plant material consists in three pools of genotypes called B, C and D, respectively corresponding to partially resistant plants (Rpv1+/Rpv2-), totally resistant plants (Rpv1-/Rpv2+), and susceptible plants (Rpv1-/Rpv2-) derived from a segregating population. Each pool consists in 3 different genotypes. Plant leaf discs were inoculated with P. viticola sporangium suspension (i) or mock-inoculated with water (ni) and analysed 6 hours post-inoculation. For each experimental condition, we performed two biological replicates. Experiment Overall Design: 3 plant genotypes were analyzed: Rpv1+/Rpv2- (B), Rpv1-/Rpv2+ (C), and Rpv1-/Rpv2- (D). Plants were either inoculated with P. viticola (i) or mock-inoculated with water (ni). Biological replicates were performed for each experimental condition.

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Phosphorylation of CAD1, PLDdelta, NDT1, RPM1 proteins induced resistance in tomatoes infected by Ralstonia solanacearum

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