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Targeted Quantification of SnRK2.6 Activation Loop Phosphorylation Status at Positions 171 and 175/176 in NIL-DOG1 and dog1-1 Dry Seeds.

ABSTRACT: Stable transgenic seeds expressing SnRK2.6 as an N-terminally tagged YFP fusion in two genetic backgrounds, NIL-DOG1 and dog1-1, were used as the starting biological material. The target protein was purified through immunoprecipitation from native dry seed crude extracts of both genotypes in three independent biological replicates. Samples were split and measured using both DDA and PRM-based methods. This assay aimed to investigate the influence of the dog1-1 mutation on the phosphorylation status of SnRK2.6 activation loops.

ORGANISM(S): Arabidopsis Thaliana (mouse-ear Cress)

SUBMITTER: IRIS FINKEMEIER

PROVIDER: PXD053457 | JPOST Repository | Thu Jun 27 00:00:00 BST 2024

REPOSITORIES: jPOST

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			Action	DRS
	240321_C24_007_OE480_PRM_GNE_2_%2324_009_GNE410.raw	Raw
	240321_C24_007_OE480_PRM_GNE_2_%2324_009_GNE411.raw	Raw
	240321_C24_007_OE480_PRM_GNE_2_%2324_009_GNE412.raw	Raw
	240321_C24_007_OE480_PRM_GNE_2_%2324_009_GNE413.raw	Raw
	240321_C24_007_OE480_PRM_GNE_2_%2324_009_GNE414.raw	Raw

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