Dataset Information

Crosslinking/mass spectrometry analysis of Fanconi anemia (FA) pathway FANCD2-FANCI complexes

ABSTRACT: DNA interstrand crosslinks (ICLs) are repaired by the Fanconi anemia (FA) pathway. The FA pathway is activated by phosphorylation of FANCI in FANCD2-FANCI complex. To investigate how phosphorylation regulates FA pathway activation and function, recombinant FANCD2-FANCI complexes prepared using either the wild-type FANCI or the phosphomimetic FANCI, were comparied using crosslinking/mass spectrometry.

ORGANISM(S): Cellular Organisms

SUBMITTER: Juri Rappsilber

PROVIDER: PXD031632 | JPOST Repository | Fri Nov 18 00:00:00 GMT 2022

REPOSITORIES: jPOST

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Dataset's files

Source:

			Action	DRS
	B210619_02_Lumos_ZC_CO_190_D2I_SDA-WT1.raw	Raw
	B210619_03_Lumos_ZC_CO_190_D2I_SDA-WT2.raw	Raw
	B210619_04_Lumos_ZC_CO_190_D2I_SDA-WT3.raw	Raw
	B210619_06_Lumos_ZC_CO_190_D2I_SDA-3D1.raw	Raw
	B210619_07_Lumos_ZC_CO_190_D2I_SDA-3D2.raw	Raw

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Publications

The DNA-damage kinase ATR activates the FANCD2-FANCI clamp by priming it for ubiquitination.

Sijacki Tamara T Alcón Pablo P Chen Zhuo A ZA McLaughlin Stephen H SH Shakeel Shabih S Rappsilber Juri J Passmore Lori A LA

Nature structural & molecular biology 20220901 9

DNA interstrand cross-links are tumor-inducing lesions that block DNA replication and transcription. When cross-links are detected at stalled replication forks, ATR kinase phosphorylates FANCI, which stimulates monoubiquitination of the FANCD2-FANCI clamp by the Fanconi anemia core complex. Monoubiquitinated FANCD2-FANCI is locked onto DNA and recruits nucleases that mediate DNA repair. However, it remains unclear how phosphorylation activates this pathway. Here, we report structures of FANCD2-F ...[more]

PMID: 36050501

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