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Priestia megaterium response to mupirocin treatment

ABSTRACT: Isoleucyl-tRNA synthetase (IleRS) is an essential enzyme that covalently couples isoleucine to the corresponding tRNA. Bacterial IleRSs group in two clades, ileS1 and ileS2, the latter bringing resistance to the natural antibiotic mupirocin. Generally, bacteria rely on either ileS1 or ileS2 as a standalone housekeeping gene. However, we have found an exception in Bacillus species: in family Bacillaceae ileS1 appears mandatory, but some of the Bacilli posses additional, ileS2 gene. Taking Priestia (Bacillus) megaterium as a model organism, we showed that PmIleRS1 is constitutively expressed, while PmIleRS2 is stress-induced. As part of our investigation of ileS1/ileS2 function, we explored the expression of ileS1 and ileS2 under control conditions and upon mupirocin treatment.

ORGANISM(S): Priestia Megaterium Nbrc 15308 = Atcc 14581

SUBMITTER: Ita Gruic-Sovulj

PROVIDER: PXD032256 | JPOST Repository | Sun Aug 28 00:00:00 BST 2022

REPOSITORIES: jPOST

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A pair of isoleucyl-tRNA synthetases in Bacilli fulfills complementary roles to keep fast translation and provide antibiotic resistance.

Zanki Vladimir V Bozic Bartol B Mocibob Marko M Ban Nenad N Gruic-Sovulj Ita I

Protein science : a publication of the Protein Society 20220901 9

Isoleucyl-tRNA synthetase (IleRS) is an essential enzyme that covalently couples isoleucine to the corresponding tRNA. Bacterial IleRSs group in two clades, ileS1 and ileS2, the latter bringing resistance to the natural antibiotic mupirocin. Generally, bacteria rely on either ileS1 or ileS2 as a standalone housekeeping gene. However, we have found an exception by noticing that Bacillus species with genomic ileS2 consistently also keep ileS1, which appears mandatory in the family Bacillaceae. Tak ...[more]

PMID: 36757682

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