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Ser/Leu-swapped cell-free translation system constructed with natural/in vitro transcribed-hybrid tRNA set

ABSTRACT: Analysis of the peptides and proteins synthesized in a cell-free translation system containing either the natural tRNA extract, the hybrid-SL tRNA set, or the hybrid-SL tRNA set minus one chimeric tRNA using LC-MS.

ORGANISM(S): Cellular Organisms

SUBMITTER: Hiroshi Murakami

PROVIDER: PXD050604 | JPOST Repository | Mon Apr 08 00:00:00 BST 2024

REPOSITORIES: jPOST

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Ser/Leu-swapped cell-free translation system constructed with natural/in vitro transcribed-hybrid tRNA set.

Fujino Tomoshige T Sonoda Ryogo R Higashinagata Taito T Mishiro-Sato Emi E Kano Keiko K Murakami Hiroshi H

Nature communications 20240516 1

The Ser/Leu-swapped genetic code can act as a genetic firewall, mitigating biohazard risks arising from horizontal gene transfer in genetically modified organisms. Our prior work demonstrated the orthogonality of this swapped code to the standard genetic code using a cell-free translation system comprised of 21 in vitro transcribed tRNAs. In this study, to advance this system for protein engineering, we introduce a natural/in vitro transcribed-hybrid tRNA set. This set combines natural tRNAs fro ...[more]

PMID: 38755134

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