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Phosphotyrosine proteomics of MDCK cell

ABSTRACT: MDCK cell with Src-overexpression (wild type or mutant) were trypsinized, and enriched with Fe-IMAC. After labelling with TMT 10plex reagent, phosphotyrosine peptides were further enriched with phosphotyrosine antibodies. The enriched peptides were analyzed with Q Exactive Plus.

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Kentaro Kajiwara, Masato Okada

PROVIDER: PXD032878 | JPOST Repository | Fri Mar 31 00:00:00 BST 2023

REPOSITORIES: jPOST

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			Action	DRS
	180216_No1_TMT_pY.raw	Raw
	txt_Maxquant.zip	Other

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Publications

Src activation in lipid rafts confers epithelial cells with invasive potential to escape from apical extrusion during cell competition.

Kajiwara Kentaro K Chen Ping-Kuan PK Abe Yuichi Y Okuda Satoru S Kon Shunsuke S Adachi Jun J Tomonaga Takeshi T Fujita Yasuyuki Y Okada Masato M

Current biology : CB 20220708 16

Abnormal/cancerous cells within healthy epithelial tissues undergo apical extrusion to protect against carcinogenesis, although they acquire invasive capacity once carcinogenesis progresses. However, the molecular mechanisms by which cancer cells escape from apical extrusion and invade surrounding tissues remain elusive. In this study, we demonstrate a molecular mechanism for cell fate switching during epithelial cell competition. We found that during competition within epithelial cell layers, S ...[more]

PMID: 35809567

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