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Shotgun Proteomics Revealed Preferential Degradation of Misfolded In Vivo Obligate GroE Substrates by Lon Protease in Escherichia coli

ABSTRACT: Proteome analysis of a conditional GroE expression strain and its variants. Proteome data for the dnaKJ-deleted strain and its variant were also included.

ORGANISM(S): Escherichia Coli

SUBMITTER: Hideki Taguchi

PROVIDER: PXD033134 | JPOST Repository | Mon Jun 27 00:00:00 BST 2022

REPOSITORIES: jPOST

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			Action	DRS
	MG1655_Ara_S1.wiff	Wiff
	MG1655_Ara_S1.wiff.scan	Wiff
	MG1655_Ara_S3.wiff	Wiff
	MG1655_Ara_S3.wiff.scan	Wiff
	MG1655_Glc_S1.wiff	Wiff

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Publications

Shotgun Proteomics Revealed Preferential Degradation of Misfolded In Vivo Obligate GroE Substrates by Lon Protease in Escherichia coli.

Niwa Tatsuya T Chadani Yuhei Y Taguchi Hideki H

Molecules (Basel, Switzerland) 20220611 12

The Escherichia coli chaperonin GroEL/ES (GroE) is one of the most extensively studied molecular chaperones. So far, ~80 proteins in E. coli are identified as GroE substrates that obligately require GroE for folding in vivo. In GroE-depleted cells, these substrates, when overexpressed, tend to form aggregates, whereas the GroE substrates expressed at low or endogenous levels are degraded, probably due to misfolded states. However, the protease(s) involved in the degradation process ...[more]

PMID: 35744894

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