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TurboID-mediated proximal labeling of Drosophila BubR1 and its cleavage variants

ABSTRACT: V5-TurboID-tagged Drosophila BubR1 and its cleavage variants were transiently expressed in Drosophila S2 cells in two biological replicates. Cells were treated with 500 µM biotin for 30 min for protein biotinylation. Biotinylated protein-containing lysate was subjected to FG-NeutrAvidin beads purification followed by on-beads trypsin digestion. Digested peptides were desalinated and purified using GL-tip SDB. Purified peptides were subjected to LC-MS/MS analysis.

ORGANISM(S): Drosophila Melanogaster (fruit Fly)

SUBMITTER: Masayuki Miura

PROVIDER: PXD033797 | JPOST Repository | Thu May 11 00:00:00 BST 2023

REPOSITORIES: jPOST

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			Action	DRS
	20211018_TurboIDBubR101.raw	Raw
	20211018_TurboIDBubR101_normalized.pdResult	Other
	20211018_TurboIDBubR102.raw	Raw
	20211018_TurboIDBubR104.raw	Raw
	20211018_TurboIDBubR105.raw	Raw

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