ABSTRACT: Collect the membrane proteins of splenic cells from C57BL6 WT and B4galt3 knockout mice activated by CD3/CD28 dynamic beads, and perform N-glycoproteomics.
Project description:RNA was prepared from papilomas of 2-3 mice (2 for WT and 3 for Knockout) and this was pooled to perform the array. Each pooled sample was run in triplicates. The mice were treated once with DMBA, followed by TPA application for 30 wks. These mice are mixed background of FVB and C57bl6 and the age when they got the papillomas was about 9 months. Keywords: Genetic modification
Project description:In order to investigate the function of Bach2 in pre-B ALL, we isolated bone marrow cells from wildtype and Bach2 knockout mice of C57Bl6 background and transformed them with BCR-ABL1.
Project description:In order to investigate the function of Bach2 in pre-B ALL, we isolated bone marrow cells from wildtype and Bach2 knockout mice of C57Bl6 background and transformed them with BCR-ABL1. We compared the gene expression profiles of Bach2 wildtype and knockout pre-B ALL cells, both with and without imatinib treatment (2uM for 16h).
Project description:To investigate the carcinogen exposure in the regulation of macrophages in the tumor microenvironment, we inoculated DMBA3-4 and DMSO3-1 cell lines in Rag1 knockout mice and collect TAMs cells. We then performed gene expression profiling analysis using data obtained from RNA-seq of tumor-associated macrophages from DMBA3-4 and DMSO3-1 groups.
Project description:We employ Mass spectrum to investigate proteome of Pum1-Knockout, Pum2-Knockout and WT conditions in human colorectal cancer cell line Hct116. Overall design: In order to investigate whether Pum1 and Pum2 regulate their targets at their protein levels, we used Pum1-Knockout, Pum2-Knockout and WT Hct116 cell line to extract total protein for Mass spectrum.
Project description:We here perform transcriptional profiling of hepatic stellate cells (HSCs) isolated from Western diet/high fructose-fed C57BL6/J mice, carbon tretrachloride (CCl4)-treated C57BL6/J mice, and of murine HSCs differentiated in vitro.
Project description:To compare gene expression between old C57BL6 female mice treated with either control lentivirus or CRISPRa+ lentivirus, we used a flurorescence-based cell sorting to collect neurons expressing GFP (from the sgRNA) from the hippocampus of the mice from each group.Then we performed gene expression analysis using data obtained from RNA-seq of the old neurons in the hippocampus of both groups.
Project description:Thymic Treg cells, mature non-Treg CD4+ single positive thymocytes, peripheral (spleen) resting and activated Treg cells were sorted from Foxp3-gfp reporter (wid type, WT) mice or Foxp3 enhancer CNS3 knockout (KO, carrying the same GFP reporter) mice. Total RNA was extracted and used for RNA sequencing to assess gene expression profiles.
Project description:Male and female knockout (KO) mice and age- and sex-matched C57BL/6J wild-type (WT) were compared. The KO mice had been backcrossed for more than 10 generations onto C57BL6/J background. The present study includes 4 strains of mice (WT, CCK1 receptor KO, CCK2 receptor KO, and CCK1+CCK2 receptor double KO). Animals that were subjected to gene expression profiling received no treatment and were killed under deep anesthesia.