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Characterization of the bovine 49-kDa subunit of respiratory complex I labeled by photoreactive acetogenin (DLA)

ABSTRACT: The proton-translocating NADH-ubiquinone oxidoreductase (complex I ) is central to mitochondrial energy metabolism. In this study, we synthesized photoreactive acetogenin mimics (DLAs) and carried out photoaffinity labeling studies using bovine heart complex I. This project contains MALDI-TOF MS data of the 49-kDa subunit, which was specifically labeled by DLAs.

ORGANISM(S): Bos Taurus

SUBMITTER: Masatoshi Murai and Hideto Miyoshi

PROVIDER: PXD041916 | JPOST Repository | Tue Apr 01 00:00:00 BST 2025

REPOSITORIES: jPOST

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Project description:Hydrogen can be an important source of energy for chemolithotrophic acidophiles, especially in the deep terrestrial subsurface. Nevertheless, the current knowledge of microbial hydrogen utilization in acidic environments is minimal. A multi-omics analysis was applied on Acidithiobacillus ferrooxidans growing aerobically and anaerobically (with ferric iron) on hydrogen as an electron donor, and a respiratory model proposed from the results obtained. In this model, both [NiFe] hydrogenases, cytoplasmic uptake and membrane-bound respiratory, oxidize molecular hydrogen to two protons and two electrons. The electrons are used to reduce membrane-soluble ubiquinone to ubiquinol. Genetically associated [FeS]-binding proteins mediate electron relay from the hydrogenases to the ubiquinone pool. Under aerobic conditions, reduced ubiquinol transfers electrons to either cytochrome aa3 oxidase via cytochrome bc1 complex and cytochrome c4 or the alternate directly to cytochrome bd oxidase, resulting in proton efflux together with the reduction of molecular oxygen to water. Under anaerobic conditions, reduced ubiquinol transfers electrons to outer membrane cytochrome c (ferric iron reductase) via cytochrome bc1 complex and a cascade of electron transporters (cytochrome c4, cytochrome c552, rusticyanin, and high potential iron-sulfur protein), resulting in proton efflux together with the reduction of ferric iron to ferrous iron. The proton gradient generated by molecular hydrogen oxidation maintains the membrane potential and allows the generation of ATP via ATP synthase and NADH via NADH-ubiquinone oxidoreductase. To a lesser extent, NADH can also be generated by another bidirectional cytoplasmic hydrogenase. ATP and NADH are further utilized in the Calvin–Benson–Bassham cycle for inorganic carbon uptake and assimilation. These results further clarify the role of extremophiles in biogeochemical processes and their impact on the composition and features of the deep terrestrial subsurface from the distant past to the present.

			Action	DRS
	1021_MSMS.mzXML	Mzxml
	1021_MSMS.txt	Txt
	1386_MSMS.mzXML	Mzxml
	1386_MSMS.txt	Txt
	2457MSMS.mzXML	Mzxml

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Characterization of the bovine 49-kDa subunit of respiratory complex I labeled by photoreactive acetogenin (DLA)

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