Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Characterization of ND5 subunit of bovine mitochondrial NADH-ubiquinone oxidoreductase (complex I)

ABSTRACT: This project contains MALDI-TOF/TOF spectra derived from the ND5 subunit of bovine mitochondrial NADH-ubiquinone oxidoreductase (complex I).

ORGANISM(S): Bos Taurus

SUBMITTER: Hideto Miyoshi

PROVIDER: PXD031179 | JPOST Repository | Fri Jul 29 00:00:00 BST 2022

REPOSITORIES: jPOST

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	1682.png	Other
	2.png	Other
	ND5-1682-msms.mzXML	Mzxml

Items per page:

1 - 3 of 3

Publications

Diverse reaction behaviors of artificial ubiquinones in mitochondrial respiratory complex I.

Uno Shinpei S Masuya Takahiro T Zdorevskyi Oleksii O Ikunishi Ryo R Shinzawa-Itoh Kyoko K Lasham Jonathan J Sharma Vivek V Murai Masatoshi M Miyoshi Hideto H

The Journal of biological chemistry 20220525 7

The ubiquinone (UQ) reduction step catalyzed by NADH-UQ oxidoreductase (mitochondrial respiratory complex I) is key to triggering proton translocation across the inner mitochondrial membrane. Structural studies have identified a long, narrow, UQ-accessing tunnel within the enzyme. We previously demonstrated that synthetic oversized UQs, which are unlikely to transit this narrow tunnel, are catalytically reduced by native complex I embedded in submitochondrial particles but not by the isolated en ...[more]

PMID: 35643318

Similar Datasets

Characterization of the Na+-NQR from V. cholerae

Project description:The Na+-pumping NADH-ubiquinone oxidoreductase (Na+-NQR) is anticipated to be a promising target of antibiotics because it is an exclusively prokaryotic enzyme and plays an essential role in bioenergetics in many pathogenic bacteria. To identify the site labeled by a ubiquinone probe, the NqrA subunit was partially digested with V8-protease. The partial digests were subjected to "in-gel" tryptic digestion, followed by MALDI-TOF MS analysis.

2022-11-19 | PXD029801 | JPOST Repository

Top-Down proteomics of unfractionated Bovine heart mitochondria

Project description:Top-Down proteomics pilot experiment of unfractionated Bovine Heart Mitochondria (BHM) using ultra high resolution Q-ToF tandem mass spectrometry (maXis 4G ETD, Bruker Daltonics).

2018-04-05 | PXD005350 | Pride

Bovine Respiratory Disease Complex

Project description:Bovine respiratory epithelial cells have different susceptibility to bovine respiratory syncytial virus infection. The cells derived from the lower respiratory tract were significantly more susceptible to the virus than those derived from the upper respiratory tract. Pre-infection with virus of lower respiratory tract with increased adherence of P. multocida; this was not the case for upper tract. However, the molecular mechanisms of enhanced bacterial adherence are not completely understood. To investigate whether virus infection regulates the cellular adherence receptor on bovine trachea-, bronchus- and lung-epithelial cells, we performed proteomic analyses.

2020-07-28 | PXD019509 | JPOST Repository

Identification of Trichuris spp. using MALDI-TOF MS

Project description:Identification of different Trichuris spp. using MALDI-TOF MS

| PRJEB50468 | ENA

Project description:Typing of Klebsiella species by FTIR and MALDI-TOF

| PRJEB27975 | ENA

A proteomic characterization reveals differences in the milk fat globule membrane of buffalo and bovine milk

Project description:The proteins of the milk fat globule membrane (MFGM) have a number of functions, such as the regulation of milk fat secretion and metabolism, the uptake and transportation of fatty acids in the intestine and potential protection from bacterial or viral infection. While the proteome of the MFGM in bovine milk has been extensively characterised, our knowledge of these proteins in buffalo milk is limited. In this study, a proteomic approach was used to characterise the proteome of the buffalo MFGM. Multiple extraction techniques were employed to increase the coverage of proteins identified, while label free relative quantitative liquid chromatography tandem mass spectrometry was used for comparison between the buffalo and bovine MFGM proteome. A total of 220 buffalo MFGM proteins and 234 bovine MFGM proteins were identified after being filtered from the initial dataset of 757 and 680 proteins respectively. Significantly greater amounts of xanthine oxidoreductase, platelet glycoprotein 4, heat shock cognate and calcineurin B homologous protein were identified per mass of buffalo MFGM protein extracted. The higher expression of xanthine oxidase in the MFGM of buffalo milk was confirmed by Western blot analysis and a heterogeneous distribution of this protein observed in situ on the surface of the MFGM. The high concentration of fat in buffalo milk, together with the differences in the MFGM proteome indicate differences in the nutritional profile, biological function, and potential ease of processing of buffalo and bovine milk products.

2017-05-19 | PXD005318 | Pride

Use of MALDI-TOF mass spectrometry and IDBac to selectively mine for understudied bacterial genera from the environment.

Project description:This bacterial MALDI-TOF dataset was used for the study "Use of MALDI-TOF mass spectrometry and IDBac to selectively mine for understudied bacterial genera from the environment'. Each spectra contains up to three replicates of MALDI-MS profiles of each bacterial isolate.

2024-06-10 | MSV000094968 | MassIVE

Triton X-100 reference proteome of brain capillary endothelial cells

Project description:Bovine brain capillary endothelial cells (BBCEC) are cocultured in presence of rat astrocytes to reinduce blood brain barrier properties. After checking of BBB propereties reinduction, BBCEC are harvested by collagenase treatment, lysed with Triton X-100 based lysis buffer. Triton X-100 soluble proteins are subjected to organic fractionation step and the 5 generated fractions were submitted to 1D-LC-MS/MS analysisi on MALDI-TOF-TOF-MS.

2010-12-03 | PRD000250 | Pride

Comparison of saliva proteins signature of three aphid species

Project description:In this study we used a multidisciplinary proteomics approach combining LC-ESI-MS/MS and 2D-DIGE/MALDI-TOF/MS to compare the salivary proteins from three aphid species including Acyrthosiphon pisum, Megoura viciae, and Myzus persicae. Comparative analyses revealed variability among aphid salivary proteomes. Among the proteins that varied 22% were related to DNA-binding, 19% were related to GTP-binding, and 19% had oxidoreductase activity.

2017-08-17 | PXD000476 | Pride

Transcriptome Characterization of Bovine Peripheral Blood Mononuclear Cells Stimulated by LPS

Project description:This study aimed to identify transcription networks and signatures of bovine peripheral blood mononuclear cells in response to LPS stimulation. A total of 464 genes including at least 17 transcription factors were identified to be significantly induced by LPS using a high density bovine oligonucleotide microarray. The network analysis revealed that alternation in gene expression was regulated by transcription factors through potential interaction within the pathway networks in the LPS stimulated cells. Our results demonstrated that specific pathway networks are responsible for transcription regulation in bovine peripheral blood mononuclear cells in response to pathogen components such as LPS.

2008-10-16 | GSE12849 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data