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Absolute quantification of mTOR related signaling molecules

ABSTRACT: To determine protein concentration in cells, we performed spike-in DIA experiments. Isotopically labeled concatemers (for mTOR-related signaling proteins) were spiked into lysates from p18Rev cells (each 20 mg) with four three-fold serial dilutions from 5,000 fmol. The lysates were subjected to digestion with trypsin. For absolute quantification of proteins, the digests containing isotopically labeled recombinant proteins were separated on a column (EV1106, Evosep) using a pre-programmed gradient (15 samples per day) at a flow rate of 200 nL/min using the Evosep One system (Evosep).

ORGANISM(S): Mus Musculus (mouse)

SUBMITTER: Masaki Matsumoto

PROVIDER: PXD042413 | JPOST Repository | Thu May 23 00:00:00 BST 2024

REPOSITORIES: jPOST

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			Action	DRS
	20211023_EVO15SPD_FAIMS-45_8uDIA_mTORconcat-250fmol.raw	Raw
	20211023_EVO15SPD_FAIMS-45_8uDIA_mTORconcat-27fmol.raw	Raw
	20211023_EVO15SPD_FAIMS-45_8uDIA_mTORconcat-83fmol.raw	Raw
	20211023_EVO15SPD_FAIMS-45_8uDIA_mTORconcat-9fmol.raw	Raw
	report.tsv	Tabular

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