Proteomics

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Proximity biotinylation of A431 cells with gefitinib alone treatment by EGFR-FabID

ABSTRACT: A431cells were pretreated with DMSO or gefitinib. Then AGIA-FabID or EGFR-FabID were added to the cells in three biological replicates. Cell lysates were subjected to methanol-chloroform precipitation and digested with trypsin. Biotinylated peptides were purified by Tamavidin 2-REV and identified by LC-MS/MS analysis.

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Hidetaka Kosako 

PROVIDER: PXD043526 | JPOST Repository | Tue Nov 14 00:00:00 GMT 2023

REPOSITORIES: jPOST

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230517_Yamada_A431_EGFR_FabID_Gefitinib_n3_Tama_FusionIT.xlsx Xlsx
334_Yamada_1_A431_AGIA_FabID_DMSO_n1_Tama_FusionIT.raw Raw
336_Yamada_1_A431_AGIA_FabID_DMSO_n2_Tama_FusionIT.raw Raw
338_Yamada_1_A431_AGIA_FabID_DMSO_n3_Tama_FusionIT.raw Raw
340_Yamada_2_A431_AGIA_FabID_Gefitinib_n1_Tama_FusionIT.raw Raw
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Publications

Proximity extracellular protein-protein interaction analysis of EGFR using AirID-conjugated fragment of antigen binding.

Yamada Kohdai K   Shioya Ryouhei R   Nishino Kohei K   Furihata Hirotake H   Hijikata Atsushi A   Kaneko Mika K MK   Kato Yukinari Y   Shirai Tsuyoshi T   Kosako Hidetaka H   Sawasaki Tatsuya T  

Nature communications 20231214 1

Receptor proteins, such as epidermal growth factor receptor (EGFR), interact with other proteins in the extracellular region of the cell membrane to drive intracellular signalling. Therefore, analysis of extracellular protein-protein interactions (exPPIs) is important for understanding the biological function of receptor proteins. Here, we present an approach using a proximity biotinylation enzyme (AirID) fusion fragment of antigen binding (FabID) to analyse the proximity exPPIs of EGFR. AirID w  ...[more]

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