Proteomics

Dataset Information

0

Proximity biotinylation of NCI-H226 cells with gefitinib alone treatment by EGFR-FabID

ABSTRACT: NCI-H226 cells were pretreated with DMSO or gefitinib. Then AGIA-FabID or EGFR-FabID were added to the cells in three biological replicates. Cell lysates were subjected to methanol-chloroform precipitation and digested with trypsin. Biotinylated peptides were purified by Tamavidin 2-REV and identified by LC-MS/MS analysis.

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Hidetaka Kosako 

PROVIDER: PXD043527 | JPOST Repository | Tue Nov 14 00:00:00 GMT 2023

REPOSITORIES: jPOST

Json Xml

Dataset's files

Source:
Action DRS
11_Yamada_1_H226_AGIAiD_DMSO_n1_Tama_FusionIT.raw Raw
13_Yamada_1_H226_AGIAiD_DMSO_n2_Tama_FusionIT.raw Raw
15_Yamada_1_H226_AGIAiD_DMSO_n3_Tama_FusionIT.raw Raw
17_Yamada_2_H226_AGIAiD_Gefitinib_n1_Tama_FusionIT.raw Raw
19_Yamada_2_H226_AGIAiD_Gefitinib_n2_Tama_FusionIT.raw Raw
Items per page:
1 - 5 of 13
altmetric image

Publications

Proximity extracellular protein-protein interaction analysis of EGFR using AirID-conjugated fragment of antigen binding.

Yamada Kohdai K   Shioya Ryouhei R   Nishino Kohei K   Furihata Hirotake H   Hijikata Atsushi A   Kaneko Mika K MK   Kato Yukinari Y   Shirai Tsuyoshi T   Kosako Hidetaka H   Sawasaki Tatsuya T  

Nature communications 20231214 1

Receptor proteins, such as epidermal growth factor receptor (EGFR), interact with other proteins in the extracellular region of the cell membrane to drive intracellular signalling. Therefore, analysis of extracellular protein-protein interactions (exPPIs) is important for understanding the biological function of receptor proteins. Here, we present an approach using a proximity biotinylation enzyme (AirID) fusion fragment of antigen binding (FabID) to analyse the proximity exPPIs of EGFR. AirID w  ...[more]

Similar Datasets

Proteomics Proximity biotinylation of A431 cells with gefitinib alone treatment by EGFR-FabID
2023-11-14 | PXD043526 | JPOST Repository
Proteomics Proximity biotinylation of A431 cells by EGFR-FabID
2023-11-14 | PXD039450 | JPOST Repository
Proteomics Proximity biotinylation of NCI-H226 cells by EGFR-FabID
2023-11-14 | PXD039451 | JPOST Repository
Proteomics Proximity biotinylation of Expi293F cells overexpressing EGFR by EGFR-FabID
2023-11-14 | PXD039449 | JPOST Repository
Proteomics Proximity biotinylation of Expi293F cells stably expressing EGFR by EGFR-FabID
2023-11-14 | PXD043525 | JPOST Repository
Transcriptomics Effect of EGFR activation on mouse chondrocytes
2019-04-24 | GSE113271 | GEO
Transcriptomics Gefitinib treatment in human EGFR-driven-Gefitinib resistant tumoral cell line
2021-07-01 | GSE117846 | GEO
Genomics Gefitinib treatment in human EGFR-driven-Gefitinib resistant tumoral cell line
| PRJNA483409 | ENA
Transcriptomics Microarray expression profile of long noncoding RNAs in EGFR-TKI resistance of lung adenocarcinoma
2016-08-23 | E-GEOD-74575 | biostudies-arrayexpress
Transcriptomics EGFR Isoforms and Gene Regulation in Human Endometrial Cancer Cells
2010-03-13 | GSE20854 | GEO