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Proteomics analysis of ALKBH8 KO mice

ABSTRACT: Proteomics analysis targeting cerebral cortex, hippocampus and cerebellum derived from AlkB homologues 8 (ALKBH8) knockout mice was conducted to identify differentially expressed proteins. Peptides were analyzed by DIA proteome analysis method using Orbitrap Eclipse, and proteins with variable expression were comprehensively analyzed by library-free method using DIA-NN.

ORGANISM(S): Mus Musculus (mouse)

SUBMITTER: Kazutake Tsujikawa

PROVIDER: PXD046196 | JPOST Repository | Fri Oct 18 00:00:00 BST 2024

REPOSITORIES: jPOST

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			Action	DRS
	20230218_ALKBH8KO_sample1.raw	Raw
	20230218_ALKBH8KO_sample10.raw	Raw
	20230218_ALKBH8KO_sample11.raw	Raw
	20230218_ALKBH8KO_sample12.raw	Raw
	20230218_ALKBH8KO_sample13.raw	Raw

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Publications

ALKBH8 contributes to neurological function through oxidative stress regulation.

Honda Kohei K Hase Hiroaki H Tanikawa Sayaka S Okawa Katsuya K Chen Lu L Yamaguchi Takumi T Nakai Manami M Kitae Kaori K Ago Yukio Y Nakagawa Shinsaku S Tsujikawa Kazutake K

PNAS nexus 20240328 3

Transfer RNA (tRNA) modification is essential for proper protein translation, as these modifications play important roles in several biological functions and disease pathophysiologies. AlkB homolog 8 (ALKBH8) is one of the nine mammalian ALKBH family molecules known to regulate selenoprotein translation through the modification of the wobble uridine (U34) in tRNA; however, its specific biological roles remain unclear. In this study, we investigated the role of ALKBH8 using <i>Alkbh8</i>-knockout ...[more]

PMID: 38550277

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