Dataset Information

The Impacts of Surgery and Intracerebral Electrodes in C57BL/6J Mouse Kainate Model of Epileptogenesis: Seizure Threshold, Proteomics, and Cytokine Profiles

ABSTRACT: ntracranial electroencephalography (EEG) is commonly used to study epileptogenesis and epilepsy in experimental models. Chronic gliosis and neurodegeneration at the injury site are known to be associated with surgically implanted electrodes in both humans and experimental models. Currently, however, there are no reports on the impact of intracerebral electrodes on proteins in the hippocampus and proinflammatory cytokines in the cerebral cortex and plasma in experimental models. We used an unbiased, label-free proteomics approach to identify the altered proteins in the hippocampus, and multiplex assay for cytokines in the cerebral cortex and plasma of C57BL/6J mice following bilateral surgical implantation of electrodes into the cerebral hemispheres.

INSTRUMENT(S): Thermo Fisher Scientific instrument model, Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Glial Cell, Hippocampal Pyramidal Layer

DISEASE(S): Epilepsy With Generalized Tonic-clonic Seizures

SUBMITTER: Maryam Sayadi

LAB HEAD: Thimmasettapp Thippeswamy

PROVIDER: PXD021554 | Pride | 2021-09-09

REPOSITORIES: Pride

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The Impacts of Surgery and Intracerebral Electrodes in C57BL/6J Mouse Kainate Model of Epileptogenesis: Seizure Threshold, Proteomics, and Cytokine Profiles.

Tse Karen K Beamer Edward E Simpson Deborah D Beynon Robert J RJ Sills Graeme J GJ Thippeswamy Thimmasettappa T

Frontiers in neurology 20210712

Intracranial electroencephalography (EEG) is commonly used to study epileptogenesis and epilepsy in experimental models. Chronic gliosis and neurodegeneration at the injury site are known to be associated with surgically implanted electrodes in both humans and experimental models. Currently, however, there are no reports on the impact of intracerebral electrodes on proteins in the hippocampus and proinflammatory cytokines in the cerebral cortex and plasma in experimental models. We used an unbia ...[more]

PMID: 34322075

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