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Site-specific SUMOylation proteomics in Arabidopsis

ABSTRACT: Identifying SUMOylation target sites in plants poses a significant challenge due to their relatively low abundance and high dynamics. Through this lysine-null SUMO1 method and the optimized two-step purification procedure, we successfully identified over 2,000 SUMOylation sites from 1,300 putative SUMO acceptors, significantly deepening the current SUMOylome in plants. Identification of thousands of SUMOylation sites offers a unique opportunity to comprehend the biological function and site specificity of SUMOylation at the proteome level.

ORGANISM(S): Arabidopsis Thaliana (mouse-ear Cress)

SUBMITTER: Tian Sang

PROVIDER: PXD047242 | JPOST Repository | Thu Sep 12 00:00:00 BST 2024

REPOSITORIES: jPOST

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			Action	DRS
	20220827_Wang_TMT_1.raw	Raw
	20220827_Wang_TMT_2.raw	Raw
	20220827_Wang_TMT_3.raw	Raw
	20220827_Wang_TMT_4.raw	Raw
	20220827_Wang_TMT_5.raw	Raw

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Site-specific SUMOylation proteomics in Arabidopsis

Project description:Identifying SUMOylation target sites in plants poses a significant challenge due to their relatively low abundance and high dynamics. Through this lysine-null SUMO1 method and the optimized two-step purification procedure, we successfully identified over 2,000 SUMOylation sites from 1,300 putative SUMO acceptors, significantly deepening the current SUMOylome in plants. Identification of thousands of SUMOylation sites offers a unique opportunity to comprehend the biological function and site specificity of SUMOylation at the proteome level.

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