Proteomics

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Proteome analysis of i-motif DNA binding proteins

ABSTRACT: Non-canonical DNA structures such as G-quadruplex (G4) and i-motif (iM) are formed at the guanine- and cytosine-rich sequences, respectively, and prohibit DNA replication and transcription. The formation and resolution of these non-canonical structures are therefore required to be dynamically regulated by either physiological conditions or factors able to bind the G4 and iM structures. Although many G4 binding proteins responsible for tuning of the G4 structure have been discovered, understanding of structural regulation of the iM structure by iM binding proteins is far less behind. Here, we developed a protein-labeling DNA probe bearing an alkyne moiety through a reactive tosylate linker for proximity labeling of nucleic acid-binding proteins and searched for iM binding proteins. The proteome analyses of the captured proteins suggested new candidates that potentially bind the iM structure, in addition to the known iM binders.

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Junpei Yamamoto 

PROVIDER: PXD051188 | JPOST Repository | Tue Oct 15 00:00:00 BST 2024

REPOSITORIES: jPOST

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20231206_Sample1_1.raw Raw
20231206_Sample1_2.raw Raw
20231206_Sample1_3.raw Raw
20231206_Sample2_1.raw Raw
20231206_Sample2_2.raw Raw
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Publications

Profiling of i-motif-binding proteins reveals functional roles of nucleolin in regulation of high-order DNA structures.

Ban Yuki Y   Ando Yuka Y   Terai Yuma Y   Matsumura Risa R   Nakane Keita K   Iwai Shigenori S   Sato Shinichi S   Yamamoto Junpei J  

Nucleic acids research 20241119

Non-canonical DNA structures, such as the G-quadruplex (G4) and i-motif (iM), are formed at guanine- and cytosine-rich sequences, respectively, in living cells and involved in regulating various biological processes during the cell cycle. Therefore, the formation and resolution of these non-canonical structures must be dynamically regulated by physiological conditions or factors that can bind G4 and iM structures. Although many G4 binding proteins responsible for tuning the G4 structure have bee  ...[more]

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