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A549 cells -- High-throughput Secretomic Analysis of Single Cells

ABSTRACT: High-throughput secretomic analysis of single A549 cells.

SUBMITTER: Rong Fan

PROVIDER: LDS-1107 | LINCS | 2015-10-06

REPOSITORIES: LINCS

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Publications

High-throughput secretomic analysis of single cells to assess functional cellular heterogeneity.

Lu Yao Y Chen Jonathan J JJ Mu Luye L Xue Qiong Q Wu Yu Y Wu Pei-Hsun PH Li Jie J Vortmeyer Alexander O AO Miller-Jensen Kathryn K Wirtz Denis D Fan Rong R

Analytical chemistry 20130201 4

Secreted proteins dictate a range of cellular functions in human health and disease. Because of the high degree of cellular heterogeneity and, more importantly, polyfunctionality of individual cells, there is an unmet need to simultaneously measure an array of proteins from single cells and to rapidly assay a large number of single cells (more than 1000) in parallel. We describe a simple bioanalytical assay platform consisting of a large array of subnanoliter microchambers integrated with high-d ...[more]

PMID: 23339603

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