Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

U87 cells -- High-throughput Secretomic Analysis of Single Cells

ABSTRACT: High-throughput secretomic analysis of single U87 cells.

SUBMITTER: Rong Fan

PROVIDER: LDS-1108 | LINCS | 2015-10-06

REPOSITORIES: LINCS

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	HMS_LINCS&file=LDS-1108.tar.gz	Other

Items per page:

1 - 1 of 1

Publications

High-throughput secretomic analysis of single cells to assess functional cellular heterogeneity.

Lu Yao Y Chen Jonathan J JJ Mu Luye L Xue Qiong Q Wu Yu Y Wu Pei-Hsun PH Li Jie J Vortmeyer Alexander O AO Miller-Jensen Kathryn K Wirtz Denis D Fan Rong R

Analytical chemistry 20130201 4

Secreted proteins dictate a range of cellular functions in human health and disease. Because of the high degree of cellular heterogeneity and, more importantly, polyfunctionality of individual cells, there is an unmet need to simultaneously measure an array of proteins from single cells and to rapidly assay a large number of single cells (more than 1000) in parallel. We describe a simple bioanalytical assay platform consisting of a large array of subnanoliter microchambers integrated with high-d ...[more]

PMID: 23339603

Similar Datasets

A549 cells -- High-throughput Secretomic Analysis of Single Cells

Project description:High-throughput secretomic analysis of single A549 cells.

2015-10-06 | LDS-1107 | LINCS

U937 cells -- High-throughput Secretomic Analysis of Single Cells

Project description:High-throughput secretomic analysis of single U937 cells.

2015-10-06 | LDS-1109 | LINCS

Characterization of differentiating adipose cells by high-throughput single-cell RNA-Seq

Project description:Directed differentiation of cells in vitro is a powerful approach for dissection of developmental pathways, disease modeling and regenerative medicine, but analysis of such systems are complicated by heterogeneous and asynchronous cellular responses to differentiation-inducing stimuli. To enable deep characterization of heterogeneous cell populations, we developed an efficient digital gene expression profiling protocol that enables surveying of mRNA in thousands of single cells at a time. We then applied this protocol to profile 11,116 cells collected during directed differentiation of human adipose-derived stem/stromal cells. The resulting data reveals the major axes of cell-to-cell variation within and between time points and suggests a link between incomplete adipogenesis in vitro and adipocyte dysfunction in vivo. High-throughput single cell RNA-seq method applied to human adipose tissue-derived stromal/stem cells during differentiation towards an adipogenic fate

2014-12-23 | E-GEOD-53638 | biostudies-arrayexpress

A high throughput pipeline for antibody validation

Project description:Western blotting (WB) is widely used to test antibody specificity, but the assay has low throughput and precision. Here we show that an inverted version of WB allows parallel readout with antibody arrays, shotgun mass spectrometry (MS) and immunoprecipitation followed by MS (IP-MS). The pipeline provides means for large-scale implementation of concepts proposed by an international working group on antibody validation (IWGAV).

2018-07-26 | PXD010510 | Pride

High-Throughput ChIPmentation

Project description:High-Throughput ChIPmentation: freely scalable, single day ChIPseq data generation from very low cell-numbers

| PRJEB23059 | ENA

High throughput gene expression analysis identifies reliable expression markers of human corneal endothelial cells.

Project description:Considerable interest has been generated for the development through cell-tissue engineering of suitable corneal endothelial graft alternatives, which can potentially alleviate the shortage of corneal transplant material. The advent of less invasive suture-less key-hole surgery options such as DescemetM-bM-^@M-^Ys Stripping Endothelial Keratoplasty (DSEK) and DescemetM-bM-^@M-^Ys Membrane Endothelial Keratoplasty (DMEK), which involve transplantation of solely the endothelial layer instead of full thickness cornea, provide further impetus for the development of alternative endothelial grafts for clinical applications. A major challenge for this endeavor is the lack of specific markers for this cell type. To identify genes that reliably mark corneal endothelial cells (CECs) in vivo and in vitro, we performed RNA-sequencing on freshly isolated human CECs (from both young and old donors), CEC cultures, and corneal stroma. Gene expression of these corneal cell types were also compared to that of other human tissue types. Based on high throughput comparative gene expression analysis, we identified a panel of markers that are: i) highly expressed in CECs from both young donors and old donors; ii) expressed in CECs in vivo and in vitro; and iii) not expressed in corneal stroma keratocytes and the activated corneal stroma fibroblasts. These were SLC4A11, COL8A2 and CYYR1. The use of this panel of genes in combination reliably ascertains the identity of the CEC cell type. A total of 20 donor corneas consisting of 10 single donor corneas and 5 paired donor corneas were used in this study. Donor age ranged from 19 - 76. This RNA-seq study included 15 pooled corneas (5 each) used form CEC old, CEC young and stroma samples.

2013-05-23 | E-GEOD-44064 | biostudies-arrayexpress

High-throughput tetrad analysis.

Project description:Tetrad analysis has been a gold-standard genetic technique for several decades. Unfortunately, the need to manually isolate, disrupt and space tetrads has relegated its application to small-scale studies and limited its integration with high-throughput DNA sequencing technologies. We have developed a rapid, high-throughput method, called barcode-enabled sequencing of tetrads (BEST), that uses (i) a meiosis-specific GFP fusion protein to isolate tetrads by FACS and (ii) molecular barcodes that are read during genotyping to identify spores derived from the same tetrad. Maintaining tetrad information allows accurate inference of missing genetic markers and full genotypes of missing (and presumably nonviable) individuals. An individual researcher was able to isolate over 3,000 yeast tetrads in 3 h, an output equivalent to that of almost 1 month of manual dissection. BEST is transferable to other microorganisms for which meiotic mapping is significantly more laborious.

| S-EPMC3696418 | biostudies-literature

High-Throughput and High-Dimensional Single Cell Analysis of Antigen-Specific CD8+ T cells

Project description:High-Throughput and High-Dimensional Single Cell Analysis of Antigen-Specific CD8+ T cells

| PRJNA732728 | ENA

High-Throughput and High-Dimensional Single Cell Analysis of Antigen-Specific CD8+ T cells

Project description:High-Throughput and High-Dimensional Single Cell Analysis of Antigen-Specific CD8+ T cells

| PRJNA732729 | ENA

MAGE-seq E. coli primary sigma factor RpoD amplicon high-throughput genotyping

Project description:Genotyping of RpoD mutants via amplicon sequencing from the following manuscript: \\"Systematic dissection of σ70 sequence diversity and function in bacteria\\" by Park and Wang (2020). Includes raw sequencing reads from samples from MAGE-seq single codon saturation mutagenesis and high-throughput fitness competition experiment as well as the RpoD ortholog mutants generated through recombineering and CRISPR selection.

2022-06-06 | E-MTAB-9103 | biostudies-arrayexpress