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IPRG 2009: Differential proteomics in Orbitrap E. coli lysates

ABSTRACT: The files in directory were generated as part of the ABRF iPRG 2009 Study. The sample is E. coli lysate. The five "red" RPLC runs lack the "green" set of proteins. The five "yellow" runs lack the "blue" set of proteins. Both red and yellow runs contain a spike of BSA to enable quantitative comparison among runs. Proteins were reduced and alkylated by DTT and iodoacetamide, respectively, and separated in a 1D gel. The proteins were trypsinized, and the peptides were added to the in-solution digest of BSA. In a collaboration between the Ayers Institute and Mass Spectrometry Research Center at Vanderbilt University Medical Center, the red and yellow samples were each separated five times in an RPLC/MS/MS experiment lasting 184 minutes (of which the shallow gradient lasted two hours ). The instrument collecting data was a Thermo LTQ-XL Orbitrap. Eight centroided tandem mass spectra were collected following each profile MS scan. Collection of these data was supported by U24 CA126479.

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Escherichia Coli (ncbitaxon:562)

SUBMITTER: David L. Tabb

PROVIDER: MSV000078539 | MassIVE | Tue Jan 28 09:18:00 GMT 2014

REPOSITORIES: MassIVE

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