Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Effect of Smoke Dye Exposure in Zebrafish Embryos

ABSTRACT: Whole transcriptome analysis of dechorinated zebrafish embryos after (static?) exposure to one of five smoke dyes from 6 hours post-fertilization (hpf) to 48 hpf. Smoke dyes tested are Disperse Blue 14, Disperse Red 9, Solvent Red 169, Solvent Violet 47, and Solvent Yellow 33. Results provide insight into the molecular mechanisms of immunological response to the dyes.

ORGANISM(S): Danio rerio

PROVIDER: GSE166302 | GEO | 2021/02/07

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Effect of Smoke Dye Exposure in Zebrafish Embryos

Project description:Effect of Smoke Dye Exposure in Zebrafish Embryos

| PRJNA700095 | ENA

Cigarette smoke induces mitochondrial metabolic reprogramming in lung cells

Project description:Cigarette smoking remains the leading cause of non-small cell lung carcinoma. Studies involving acute exposure of smoke on lung cells revealed induction of pre- cancerous state in lung cells. Recently few studies have reported the chronic effect of cigarette smoke in inducing cellular transformation. Yet no systemic study has been performed to understand the molecular alterations in lung cells due to cigarette smoke. Hence it is both important and necessary to study the chronic effect of cigarette smoke in a temporal setting to understand the molecular alterations. In this study, we carried out TMT based proteomic profiling of lung cells which were exposed to cigarette smoke condensate (CSC) for upto 12 months. We identified 2621 proteins in total, of which 145, 114, 87, 169 and 671 proteins were differentially expressed (p<0.05, 1.5 fold) in 2nd, 4th, 6th, 8th and 12th month respectively. Pathway analysis revealed enrichment of xenobiotic metabolism signaling for the first 8 months of smoke treatment, where as continued exposure of smoke for 12 months revealed mitochondrial reprogramming in cells which includes dysregulation of oxidative phosphorylation machinery leading to enhanced reactive oxygen species and higher expression of enzymes involved in tricarboxylic acid cycle (TCA). In addition, chronic exposure of smoke led to overexpression of enzymes involved in glutamine metabolism, fatty acid degradation and lactate synthesis. This could possibly explain the availability of alternative source of carbon in TCA cycle apart from glycolytic pyruvate. Our data indicates that chronic exposure to cigarette smoke induces mitochondrial metabolic transformation in cells to support growth and survival.

2022-03-02 | PXD007108 | Pride

Wildfire Smoke-Induced MicroRNA Changes in Mouse Plasma Extracellular Vesicles

Project description:These analyses set out to evaluate changes in microRNA signatures in the mouse plasma in response to simulated wildland fire exposure conditions. These exposure conditions were based on smoke condensate consisting of particulate matter and semivolative organic compounds resulting from the burning of two biomass fuels; namely , peat and red oak. These biomasses were evaluated under two burn conditions: flaming and smoldering. Saline exposures were also carried out as negative controls.

2022-09-01 | GSE173561 | GEO

Wildfire Smoke-Induced Transcriptomic Changes in the Mouse Lung and Heart

Project description:These analyses set out to evaluate transcriptomic responses in the mouse lung and heart in response to simulated wildland fire exposure conditions. These exposure conditions were based on smoke condensate consisting of particulate matter and semivolative organic compounds resulting from the burning of five biomass fuels; namely eucalyptus, pine, pine needles, peat, and red oak. These biomasses were evaluated under two burn conditions: flaming and smoldering.

2022-09-01 | GSE164542 | GEO

Dye Bias Array Set

Project description:Spot intensity serves as a proxy for gene expression in dual-label microarray experiments. Dye bias is defined as an intensity difference between samples labeled with different dyes attributable to the dyes instead of the gene expression in the samples. Dye bias that is not removed by array normalization can introduce bias into comparisons between samples of interest. But if the bias is consistent across the samples for the same gene, it can be corrected by proper experimental design and analysis. If the dye bias is not consistent across samples for the same gene, but is different for different samples, then removing the bias becomes more problematic, perhaps indicating a technical limitation to the ability of fluorescent signals to accurately represent gene expression. Thus, it is important to characterize dye bias to determine: (1) whether it will be removed for all genes by array normalization, (2) whether it will not be removed by normalization but can be removed by proper experimental design and analysis and (3) whether dye bias correction is more problematic than either of these and is not easily removable. Keywords: dye swap design

2006-06-06 | GSE4982 | GEO

Development of a novel culture medium for embryo transfer using a dye library.

Project description:The effects of dyes that enhance visibility during embryo transfer on embryonic development were analyzed.

2024-05-28 | GSE268174 | GEO

NHBE exposed to cigarette smoke

Project description:normal human bronchial epithelial cultures from two cultures in parallel exposed to cigarette smoke (CS) or air (mock) at timepoints 4 hours and 24 hours. Keywords = cigarette smoke Keywords = microarray Keywords = bronchial cell Keywords = tobacco Keywords: time-course

2005-02-23 | GSE2302 | GEO

Kentucky assay: cigarette smoke

Project description:Kentucky assay: cigarette smoke

| PRJNA626542 | ENA

mouse lung resistance or sensitivity to cigarette smoke

Project description:We have investigated the effects of cigarette smoke exposure in three different strains of mice. DBA/2 and C57Bl/6J are susceptible to smoke and develop different lung changes in response to chronic exposure, while ICR mice are resistant to smoke and do not develop emphysema. The present study was carried out to determine early changes in the gene expression profile of mice exposed to cigarette smoke with either a susceptible or resistant phenotype.

2008-07-10 | GSE12036 | GEO

Transcriptional profiling of Regulatory T cells isolated from mice exposed to Smoke, Morphine and Smoke plus Morphine

Project description:Purpose: The goals of this study were to identify gene expression signatures that are unique to Tregs from mice upon treatments with smoke, morphine and smoke plus morphine Methods and results: Wild-type mice were treated with cigarette smoke (SM) for a period of eight weeks, and/or the chronic continuous administration of morphine (M) via mini-pumps for the final four weeks CD4+CD25highCD127low Tregs from spleen were purified by cell sorting to generate mRNA transcription. Transcriptional profiling revealed a unique Treg signatures in SM, M or SM + M relative to control mice. Conclusion: Our study represents the first detailed analysis of splenic Treg transcriptome in mice exposure to smoke plus morphine.

2022-04-14 | GSE198210 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data