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Billon et al., PCNA acetylation dataset

ABSTRACT: This submission contains the mass spectrometry files for the manuscript by Billon et al. that identified key lysines residues within PCNA that control its functions. This submission contains 16 .raw and 16.dta files where a gel band containing PCNA was analyzed as well as 2 scaffold files used for spectra visualization. See the README file within "Methods and Protocols" and the accompanying File description.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Anne-Claude Gingras

PROVIDER: MSV000078851 | MassIVE |

REPOSITORIES: MassIVE

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Publications

Acetylation of PCNA Sliding Surface by Eco1 Promotes Genome Stability through Homologous Recombination.

Billon Pierre P Li Jian J Lambert Jean-Philippe JP Chen Yizhang Y Tremblay Véronique V Brunzelle Joseph S JS Gingras Anne-Claude AC Verreault Alain A Sugiyama Tomohiko T Couture Jean-Francois JF Côté Jacques J

Molecular cell 20161201 1

During DNA replication, proliferating cell nuclear antigen (PCNA) adopts a ring-shaped structure to promote processive DNA synthesis, acting as a sliding clamp for polymerases. Known posttranslational modifications function at the outer surface of the PCNA ring to favor DNA damage bypass. Here, we demonstrate that acetylation of lysine residues at the inner surface of PCNA is induced by DNA lesions. We show that cohesin acetyltransferase Eco1 targets lysine 20 at the sliding surface of the PCNA ...[more]

PMID: 27916662

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