Proteomics

Dataset Information

0

Proteomics reveals dynamic assembly of repair complexes during bypass of DNA cross-links part 2 of 2: AP-MS DATA (QUBIC)


ABSTRACT: DNA interstrand crosslinks (ICLs) block replication fork progression by inhibiting DNA strand separation. Repair of ICLs requires sequential incisions, translesion DNA synthesis, and homologous recombination, but the full set of factors involved in these transactions remains unknown. Here we established CHROmatin MASS spectrometry (CHROMASS) to study protein recruitment dynamics during perturbed DNA replication in Xenopus egg extracts. Using CHROMASS, we systematically monitored protein assembly and disassembly at ICL-containing chromatin. Among numerous prospective new DNA repair factors we identified SLF1 and SLF2, which form a complex with RAD18 and together define a new pathway that suppresses genome instability by recruiting the SMC5/6 cohesion complex to DNA lesions. Our study provides the first global analysis of an entire DNA repair pathway and reveals the mechanism of SMC5/6 relocalization to damaged DNA in vertebrate cells.

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Dr Matthias Mann 

PROVIDER: MSV000079577 | MassIVE | Sun Mar 13 12:00:00 GMT 2016

SECONDARY ACCESSION(S): PXD000491

REPOSITORIES: MassIVE

Dataset's files

Source:
Action DRS
Other
Items per page:
1 - 1 of 1
altmetric image

Publications


DNA interstrand cross-links (ICLs) block replication fork progression by inhibiting DNA strand separation. Repair of ICLs requires sequential incisions, translesion DNA synthesis, and homologous recombination, but the full set of factors involved in these transactions remains unknown. We devised a technique called chromatin mass spectrometry (CHROMASS) to study protein recruitment dynamics during perturbed DNA replication in Xenopus egg extracts. Using CHROMASS, we systematically monitored prote  ...[more]

Similar Datasets

2015-05-07 | PXD000490 | Pride
2015-05-07 | PXD000491 | Pride
2024-02-24 | E-MTAB-12912 | biostudies-arrayexpress
2024-08-06 | PXD043107 | Pride
2020-03-09 | PXD017020 | Pride
2024-02-12 | PXD044310 | Pride
2019-11-08 | PXD006741 | Pride
2017-02-02 | PXD004828 | Pride
2024-06-19 | PXD045353 | Pride
2022-02-22 | PXD031168 | Pride