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Diffacto quantification-centered large-scale differential analysis

ABSTRACT: In comparative shotgun proteomics, the objects of measurements are proteolytic peptides, while the objects of interest are proteins. The transition from the measured peptide abundances to the presumed protein expression levels is nontrivial and has not been sufficiently studied. With our new approach named Diffacto, we aim to improve peptide-to-protein transition utilizing the high statistical power of signal correlations in large-scale proteomics datasets. Diffacto detects and removes unreliably measured quantities while aggregating peptide-level information, which makes protein inference and quantification more accurate and robust.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Bsa Saccharomyces Cerevisiae (ncbitaxon:4932) Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Roman Zubarev and Lukas K�ll

PROVIDER: MSV000079811 | MassIVE | Sat Jun 11 07:37:00 BST 2016

SECONDARY ACCESSION(S): PXD004308

REPOSITORIES: MassIVE

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