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Bovine tissue and blood contamination from bullets by LC-MS/MS

ABSTRACT: We have measured the proteomes of different major bovine organs in multiple replicate. Before the actual sample preparation, organs were penetrated with metal bullets experimentally. The biological material on these projectiles was then "on-surface" digested using trypsin and analyzed using LC-MS/MS. Further experiments have been done testing the influence of different metal types, shooting and the contamination with human blood.

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Bos Taurus (ncbitaxon:9913) Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Dr Sascha Dammeier

PROVIDER: MSV000080090 | MassIVE | Tue Aug 23 00:39:00 BST 2016

SECONDARY ACCESSION(S): PXD002193

REPOSITORIES: MassIVE

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Publications

Mass-Spectrometry-Based Proteomics Reveals Organ-Specific Expression Patterns To Be Used as Forensic Evidence.

Dammeier Sascha S Nahnsen Sven S Veit Johannes J Wehner Frank F Ueffing Marius M Kohlbacher Oliver O

Journal of proteome research 20151208 1

Standard forensic procedures to examine bullets after an exchange of fire include a mechanical or ballistic reconstruction of the event. While this is routine to identify which projectile hit a subject by DNA analysis of biological material on the surface of the projectile, it is rather difficult to determine which projectile caused the lethal injury--often the crucial point with regard to legal proceedings. With respect to fundamental law it is the duty of the public authority to make every end ...[more]

PMID: 26593679

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Bovine tissue and blood contamination from bullets by LC-MS/MS

Project description:We have measured the proteomes of different major bovine organs in multiple replicate. Before the actual sample preparation, organs were penetrated with metal bullets experimentally. The biological material on these projectiles was then "on-surface" digested using trypsin and analyzed using LC-MS/MS. Further experiments have been done testing the influence of different metal types, shooting and the contamination with human blood.

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Project description:In cattle, almost all fully grown vesicle stage oocytes (GV) have the ability to resume meisos, develop to Metaphase II stage (MII), support fertilization and progress through the early embryonic cycles in vitro. Yet without intensive selection, the majority fail to develop to the blastocyst stage. Using the Affymetrix Bovine Genome Array, global mRNA expression analysis of immature (GV) and in vitro matured (IVM) bovine oocytes was carried out to characterize the transcriptome of the bovine oocyte and to identify the key pathways associated with oocyte meiotic maturation and developmental potential. Immature and in vitro matured bovine oocytes were collected for RNA extraction and hybridization on Affymetrix GeneChip Bovine Genome array. Careful removal of cumulus and selection of oocytes was carried out under the stereo microscope in order to examine the actual cumulus-free temporal oocyte gene expression profiles. Immature oocytes at time 0 h and in vitro matured oocytes at 24 h were collected for analysis.

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Response of bovine mammary tissue to experimental Staphylococcus aureus infection

Project description:Mastitis in dairy cattle can result from infection by a range of microorganisms but is principally caused by coliform bacteria and gram positive bacteria such as Staphylococcus aureus (S. aureus). The former species are often acquired by environmental contamination while S. aureus is particularly problematic due to its resistance to antibiotic treatments and ability to reside within mammary tissue in a chronic, subclinical state. The transcriptional and translational responses within bovine mammary epithelial tissue subjected to intramammary challenge with S. aureus are poorly characterised, particularly at the earliest stages of infection. A Bovine Innate Immune Microarray was employed to measure changes in gene expression occurring in bovine mammary tissues sampled from three dairy cows after a brief and graded intramammary challenge with a virulent strain of S. aureus. This SuperSeries is composed of the SubSeries listed below.

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Study of microbial functional structure in Xiangjiang River sediment

Project description:Xiangjiang River (Hunan, China) has been contaminated with heavy metal for several decades by surrounding factories. However, little is known about the influence of a gradient of heavy metal contamination on the diversity, structure of microbial functional gene in sediment. To deeply understand the impact of heavy metal contamination on microbial community, a comprehensive functional gene array (GeoChip 5.0) has been used to study the functional genes structure, composition, diversity and metabolic potential of microbial community from three heavy metal polluted sites of Xiangjiang River.

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Response of bovine mammary tissue to experimental Staphylococcus aureus infection experiment 3.

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Response of bovine mammary tissue to experimental Staphylococcus aureus infection experiment 2.

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Response of bovine mammary tissue to experimental Staphylococcus aureus infection experiment 1.

2010-05-20 | GSE9628 | GEO

Response of bovine mammary tissue to experimental Staphylococcus aureus infection experiment 3.

Project description:Mastitis in dairy cattle can result from infection by a range of microorganisms but is principally caused by coliform bacteria and gram positive bacteria such as Staphylococcus aureus (S. aureus). The former species are often acquired by environmental contamination while S. aureus is particularly problematic due to its resistance to antibiotic treatments and ability to reside within mammary tissue in a chronic, subclinical state. The transcriptional and translational responses within bovine mammary epithelial tissue subjected to intramammary challenge with S. aureus are poorly characterised, particularly at the earliest stages of infection. A Bovine Innate Immune Microarray was employed to measure changes in gene expression occurring in bovine mammary tissues sampled from three dairy cows after a brief and graded intramammary challenge with a virulent strain of S. aureus. Keywords: dose response, disease state analysis Animal infected with vaying doses of S. aureus in different udder locations were compared to a single control uninfected tissue and to the other 2 locations at each infective dose. Dye swaps were performed for each comparison.

2010-05-20 | E-GEOD-9681 | biostudies-arrayexpress

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